Nitrotyrosine Antibody (39B6) [Allophycocyanin/Cy7]

Novus Biologicals, part of Bio-Techne | Catalog # NB110-96877APCCY7

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Rat, Non-species specific

Applications

ELISA, Immunocytochemistry/ Immunofluorescence

Label

Allophycocyanin/Cy7 (Excitation = 650;755 nm, Emission = 767 nm)

Antibody Source

Monoclonal Mouse IgG2A Clone # 39B6

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Nitrotyrosine Antibodies »

Product Specifications

Immunogen

3-(4-hydroxy-3-nitrophenylacetamido) propionic acid-bovine serum albumin

Reactivity Notes

Species Independent. Rat reactivity reported in scientific literature (PMID:32980492)

Specificity

Recognizes 3-nitrotyrosine moieties. No detectable cross-reactivity with non-nitrated tyrosine. Not species specific.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Applications for Nitrotyrosine Antibody (39B6) [Allophycocyanin/Cy7]

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined. For optimal results using our Tandem dyes, please avoid prolonged exposure to light or extreme temperature fluctuations. These can lead to irreversible degradation or decoupling. When staining intracellular targets, specific attention to the fixation and permeabilization steps in your flow protocol may be required. Please contact our technical support team at technical@novusbio.com if you have any questions.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: Nitrotyrosine

Protein tyrosine nitration results in a post-translational modification that is increasingly receiving attention as an important component of nitric oxide signaling (2). While multiple nonenzymatic mechanisms are known to be capable of producing nitrated tyrosine residues, most tyrosine nitration events involve catalysis by metalloproteins such as myeloperoxidase, eosinophilperoxidase (3), myoglobin, the cytochrome P-450s, superoxide dismutase and prostacyclin synthase. Nitrotyrosine may also serve as a biomarker for the effects of reactive nitrogen oxides, based on tyrosine residues becoming nitrated in proteins at sites of inflammation induced tissue injury (1). The presence of nitro tyrosine-containing proteins therefore has shown high correlation to disease states such as atherosclerosis, Alzheimer's disease, Parkinson's disease and amyotrophic lateral sclerosis (4).

Alternate Names

Nitrotyrosine

Additional Nitrotyrosine Products

Product Documents for Nitrotyrosine Antibody (39B6) [Allophycocyanin/Cy7]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Nitrotyrosine Antibody (39B6) [Allophycocyanin/Cy7]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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