Nitrotyrosine Antibody (EM-30) [DyLight 550]

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-96130R

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Non-species specific

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

DyLight 550 (Excitation = 562 nm, Emission = 576 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # EM-30

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Nitrotyrosine Antibodies »

Product Specifications

Immunogen

NO2-Tyr-CH2-Thyroglobulin

Reactivity Notes

This antibody reacts to large number of species. Use in Mouse reported in secitific publication PMID: 32793221

Specificity

The antibody EM-30 detects nitrotyrosine group in biological material. It can be used as a marker for peroxynitrite formation in particular tissues

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for Nitrotyrosine Antibody (EM-30) [DyLight 550]

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

50mM Sodium Borate

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Nitrotyrosine

Protein tyrosine nitration results in a post-translational modification that is increasingly receiving attention as an important component of nitric oxide signaling (2). While multiple nonenzymatic mechanisms are known to be capable of producing nitrated tyrosine residues, most tyrosine nitration events involve catalysis by metalloproteins such as myeloperoxidase, eosinophilperoxidase (3), myoglobin, the cytochrome P-450s, superoxide dismutase and prostacyclin synthase. Nitrotyrosine may also serve as a biomarker for the effects of reactive nitrogen oxides, based on tyrosine residues becoming nitrated in proteins at sites of inflammation induced tissue injury (1). The presence of nitro tyrosine-containing proteins therefore has shown high correlation to disease states such as atherosclerosis, Alzheimer's disease, Parkinson's disease and amyotrophic lateral sclerosis (4).

Alternate Names

Nitrotyrosine

Additional Nitrotyrosine Products

Product Documents for Nitrotyrosine Antibody (EM-30) [DyLight 550]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Nitrotyrosine Antibody (EM-30) [DyLight 550]



DyLight (R) is a trademark of Thermo Fisher Scientific Inc. and its subsidiaries.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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