Nitrotyrosine Antibody (HM.11)
Novus Biologicals, part of Bio-Techne | Catalog # NB600-1024
Key Product Details
Species Reactivity
Human
Applications
Immunoassay, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2B Clone # HM.11
Concentration
0.1 mg/ml
Product Specifications
Immunogen
Nitrated KLH
Specificity
This highly specific monoclonal antibody HM.11 reacts with nitrotyrosine, both with the free amino acid as well as with proteins containing nitrotyrosine. The presence of nitrotyrosine has been detected in various inflammatory processes including atherosclerotic plaques. Nitrotyrosine is formed in tissues in presence of the active metabolite NO and is a stable end product of nitrosylation of tyrosine. Various pathways including the formation of peroxinitrite lead to nitrotyrosine production.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2B
Scientific Data Images for Nitrotyrosine Antibody (HM.11)
![Immunohistochemistry-Paraffin: Nitrotyrosine Antibody (HM.11) [NB600-1024]](https://resources.bio-techne.com/images/products/Nitrotyrosine-Antibody-HM11-Immunohistochemistry-Paraffin-NB600-1024-img0003.jpg "Immunohistochemistry-Paraffin: Nitrotyrosine Antibody (HM.11) [NB600-1024]")
Immunohistochemistry-Paraffin: Nitrotyrosine Antibody (HM.11) [NB600-1024]
Immunohistochemistry-Paraffin: Nitrotyrosine Antibody (HM11) [NB600-1024] - Analysis using the Biotin conjugate of NB600-1024. Nitrotyrosine in human liver of severely obesed patients. Staining of paraffin tissue section with clone HM.11. Anti-nitrotyrosine at 2 ug/ml (1h, RT).Applications for Nitrotyrosine Antibody (HM.11)
Application
Recommended Usage
Immunohistochemistry
1:10-1:500
Immunohistochemistry-Frozen
1:10-1:500
Immunohistochemistry-Paraffin
1:10-1:500
Western Blot
1:100-1:2000
Application Notes
Nitrotyrosine is a stable end product of nitrosylation of tyrosine and can be detected by histology on paraffin sections and frozen sections and by Western blot. The antibody is suitable for ELISA applications. (Warning: keep sterile, do not add sodium azide or any nitric containing preservative since this will affect the antibody)
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
0.2 um filtered solution in PBS and 0.1% BSA
Preservative
No Preservative
Concentration
0.1 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C.
Background: Nitrotyrosine
Alternate Names
Nitrotyrosine
Additional Nitrotyrosine Products
Product Documents for Nitrotyrosine Antibody (HM.11)
Product Specific Notices for Nitrotyrosine Antibody (HM.11)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...