NK1R Antibody - BSA Free

Western Blot: NK1R AntibodyBSA Free [NB300-119]

Western Blot: NK1R Antibody [NB300-119] - Total protein from human, mouse and rat brain was separated on a 12% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% non-fat milk in TBST. The membrane was probed with 2.0 ug/mL anti-NK1R in 1% non-fat milk in TBST and detected with an anti-rabbit HRP secondary antibody using chemiluminescence.

Western Blot: NK1R AntibodyBSA Free [NB300-119]

Western Blot: NK1R Antibody [NB300-119] - TACR1 and its downstream targets SRC and p-SRC are expressed in neuroblastoma cell lines. Endogenous TACR1 (NK1R), SRC and p-SRC expression in whole-cell extracts of neuroblastoma cell lines was visualized by western blotting. GAPDH was used as a loading control. Image collected and cropped by CiteAb from the following publication (https://www.oncotarget.com/lookup/doi/10.18632/oncotarget.13440), licensed under a CC-BY license.

Immunocytochemistry/Immunofluorescence: NK1R Antibody [NB300-119] -

Immunocytochemistry/Immunofluorescence: NK1R Antibody [NB300-119] - Overnight incubation at 4C with NK1R antibody diluted 1:100 in PBS/BSA 1%. Red: Phalloidin, Blue: DAPI, Green: NK1R antibody. Image from verified customer review.

Western Blot: NK1R Antibody - BSA Free [NB300-119] -

Western Blot: NK1R Antibody - BSA Free [NB300-119] - TACR1 & its downstream targets SRC & p-SRC are expressed in neuroblastoma cell lines(A) Endogenous TACR1, SRC & p-SRC expression in whole-cell extracts of neuroblastoma cell lines was visualized by western blotting. GAPDH was used as a loading control. (B) Quantitative RT-PCR of TACR1 mRNA in a panel of neuroblastoma cell lines (n = 3, error bars indicate standard deviation). (C–E) Quantification of SRC (C), p-SRC (D) & TACR1 (E) protein expression using densitometry analysis of western immunoblots (n = 3, error bars indicate standard deviation). Image collected & cropped by CiteAb from the following publication (https://www.oncotarget.com/lookup/doi/10.18632/oncotarget.13440), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: NK1R Antibody - BSA Free [NB300-119] -

Western Blot: NK1R Antibody - BSA Free [NB300-119] - Striatal beta3 subunit protein reduced conditional KO mice. (A) Fluorescent images demonstrating colocalization between endogenous tdTomato fluorescence & DARPP-32 expression. Approximately half of the DARPP-32+ cells expressed endogenous tdTomato, indicative of Cre expression. Scale bar is 25 μm. (B) Representative western blot analysis of the GABAA receptor beta3 subunit from individual 30-day-old mice revealed reduced amounts of beta3 subunit protein in striatum (B) from Cre positive ( beta3f/fDrd2) animals compared to Cre negative ( beta3f/+ & beta3f/f) control animals. The amount of beta3 protein in cortex (C) did not differ between genotypes. Blots were reprobed for beta-actin. (D) Summary graph of western blot analysis demonstrating a significant reduction in beta3 protein in striatum, but not cortex. Data are expressed as percent change in band intensity relative to Cre negative controls following normalization to actin. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/21847370), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: NK1R Antibody - BSA Free [NB300-119] -

Western Blot: NK1R Antibody - BSA Free [NB300-119] - Striatal beta3 subunit protein reduced conditional KO mice. (A) Fluorescent images demonstrating colocalization between endogenous tdTomato fluorescence & DARPP-32 expression. Approximately half of the DARPP-32+ cells expressed endogenous tdTomato, indicative of Cre expression. Scale bar is 25 μm. (B) Representative western blot analysis of the GABAA receptor beta3 subunit from individual 30-day-old mice revealed reduced amounts of beta3 subunit protein in striatum (B) from Cre positive ( beta3f/fDrd2) animals compared to Cre negative ( beta3f/+ & beta3f/f) control animals. The amount of beta3 protein in cortex (C) did not differ between genotypes. Blots were reprobed for beta-actin. (D) Summary graph of western blot analysis demonstrating a significant reduction in beta3 protein in striatum, but not cortex. Data are expressed as percent change in band intensity relative to Cre negative controls following normalization to actin. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/21847370), licensed under a CC-BY license. Not internally tested by Novus Biologicals.