NKCC2/SLC12A1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12228

Novus Biologicals, part of Bio-Techne
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NBP3-12228
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Primate

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all NKCC2/SLC12A1 Antibodies »

Product Summary for NKCC2/SLC12A1 Antibody

Immunogen

Synthetic peptide taken within amino acid region 30-60 on NKCC2 protein.

Clonality

Polyclonal

Host

Rabbit

Scientific Data Images for NKCC2/SLC12A1 Antibody

Immunohistochemistry-Paraffin: NKCC2/SLC12A1 Antibody [NBP3-12228]

Immunohistochemistry-Paraffin: NKCC2/SLC12A1 Antibody [NBP3-12228]

Immunohistochemistry-Paraffin: NKCC2/SLC12A1 Antibody [NBP3-12228] - Rat stomach-NBP3-12228. 1:50 dilution in IHC blocking buffer. DAB (brown) staining and hematoxylin QS (blue) counterstrain. 40x magnification on leica DM4000
Immunohistochemistry-Paraffin: NKCC2/SLC12A1 Antibody [NBP3-12228]

Immunohistochemistry-Paraffin: NKCC2/SLC12A1 Antibody [NBP3-12228]

Immunohistochemistry-Paraffin: NKCC2/SLC12A1 Antibody [NBP3-12228] - Rat stomach. 1:150 dilution IHC blocking buffer. DAB (brown) staining and Hematoxylin QS (blue) counterstain. 10X, 40X magnification.

Applications for NKCC2/SLC12A1 Antibody

Application
Recommended Usage

ELISA

1:10000

Western Blot

1:500
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

Contains Tris, HCl/glycine buffer (pH 7.4-7.8), 30% glycerol, 0.5% BSA, along with cryo-protective agents, and HEPES (exact storage and stabilization buffer information is proprietary).

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Store at -20C long term. Avoid freeze-thaw cycles.

Background: NKCC2/SLC12A1

The sodium-potassium-chloride cotransporter isoform 2 is kidney-specific and is found on the apical membrane of the thick ascending limb of Henle's loop and the macula densa. It accounts for most of the NaCl resorption with the stoichiometry of 1Na:1K:2Cl and is sensitive to such diuretics as furosemide and bumetanide. Some Bartter-like syndromes result from defects in this gene. [provided by RefSeq]

Long Name

Sodium/Potassium/Chloride Transporter NKCC2

Alternate Names

BSC1, SLC12A1

Gene Symbol

SLC12A1

Additional NKCC2/SLC12A1 Products

Product Documents for NKCC2/SLC12A1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for NKCC2/SLC12A1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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