NKp30/NCR3 Antibody (012)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-89525

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 012

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all NKp30/NCR3 Antibodies »

Product Specifications

Immunogen

This antibody was obtained from a rabbit immunized with purified, recombinant Human NKp30/NCR3 (Accession#: NP_667341.1; Met1-Gly135).

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Description

This antibody can be stored at 2C-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.

Scientific Data Images for NKp30/NCR3 Antibody (012)

Western Blot: NKp30/NCR3 Antibody (012) [NBP2-89525]

Western Blot: NKp30/NCR3 Antibody (012) [NBP2-89525]

Western Blot: NKp30/NCR3 Antibody (012) [NBP2-89525] - Lane A: K562 Whole Cell Lysate Lysates/proteins at 30 ug per lane.Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:22 kDa Observed band size:14 kDa

Applications for NKp30/NCR3 Antibody (012)

Application
Recommended Usage

ELISA

1:25000-1:50000

Western Blot

1:500-1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 um filtered solution in PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NKp30/NCR3

NKp30, also referred to as natural cytotoxicity receptor 3 (NCR3), is a type I transmembrane receptor belonging to the immunoglobulin (Ig) superfamily that is expressed on resting and active natural killer (NK) cells with a role in triggering a cytotoxic response against tumor cells (1). The human NKp30 protein has six isoforms. The canonical isoform (NKp30a) is 177 amino acids (aa) in length with a theoretical molecular weight of 21.6 kDa (1,2). The NKp30 protein consists of a signal peptide, an Ig domain, a stalk region, a transmembrane domain, and an intracellular region (1-3). NKp30/NCR3 signaling relies on interaction with the immunoreceptor tyrosine-based activation motif (ITAM)-containing adaptor molecules CD3zeta and FcRgamma (1,4,5). Other NRC family members include NKp44 and NKp46 (1,3-6). NK cell activation and tumor surveillance occurs through receptor-ligand binding. Tumor-derived ligands for NKp30 include HLA-B-associated transcript 3 (BAT3)/Bcl2-associated anthogene 6 (BAG6) and B7-H6 (4-6). NKp30 has also been suggested to play a role in NK-dendritic cell (DC) crosstalk by promoting DC maturation and cytotoxicity of immature DCs, mediated in part by BAT3 (1,4,5).

Given its role in NK cell-mediated killing and activation, NKp30 is a potential target for cancer immunotherapies (6). For instance, Bi-specific T cell engagers (BiTE) is one immunotherapy approach targeting the NKp30/B7-H6 interaction where B7-H6-specific BiTE elicits an attack on tumor cells through interferon gamma (IFNgamma) production and T cell cytotoxicity (1). Another potential approach is NKp30-derived chimeric antigen receptors (CARs) where CAR-T cells that express chimeric NKp30 receptors can efficiently attack B7-H6 tumor cells (1,6).

References

1. Barrow, A. D., Martin, C. J., & Colonna, M. (2019). The natural cytotoxicity receptors in health and disease. Frontiers in Immunology, 10, 909. https://doi.org/10.3389/fimmu.2019.00909

2. Uniprot (O14931)

3. Luczo, J. M., Ronzulli, S. L., & Tompkins, S. M. (2021). Influenza A virus hemagglutinin and other pathogen glycoprotein interactions with NK cell natural cytotoxicity receptors NKp46, NKp44, and NKp30. Viruses, 13(2), 156. https://doi.org/10.3390/v13020156

4. Pazina, T., Shemesh, A., Brusilovsky, M., Porgador, A., & Campbell, K. S. (2017). Regulation of the Functions of natural cytotoxicity receptors by interactions with diverse ligands and alterations in splice variant expression. Frontiers in Immunology, 8, 369.

5. Kaifu, T., Escaliere, B., Gastinel, L. N., Vivier, E., & Baratin, M. (2011). B7-H6/NKp30 interaction: a mechanism of alerting NK cells against tumors. Cellular and Molecular Life Sciences : CMLS, 68(21), 3531-3539. https://doi.org/10.1007/s00018-011-0802-7

6. Pinheiro, P. F., Justino, G. C., & Marques, M. M. (2020). NKp30 - A prospective target for new cancer immunotherapy strategies. British Journal of Pharmacology, 177(20), 4563-4580. https://doi.org/10.1111/bph.15222

Alternate Names

CD337, NCR3

Gene Symbol

NCR3

Additional NKp30/NCR3 Products

Product Documents for NKp30/NCR3 Antibody (012)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for NKp30/NCR3 Antibody (012)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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