NKX2.5 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-24675

Novus Biologicals, part of Bio-Techne
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NBP2-24675
NBP2-24675SS
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Key Product Details

Species Reactivity

Validated:

Human, Primate

Predicted:

Bovine (100%), Chimpanzee (100%), Equine (100%), Feline (100%), Porcine (100%), Rhesus Macaque (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.5 mg/ml

View all NKX2.5 Antibodies »

Product Specifications

Immunogen

A portion of amino acids 300-324 of human NKX2-5 was used as the immunogen.

Reactivity Notes

The amino acid sequence used as immunogen is 88% homologous in mouse, rat and opossum.

Marker

Cardiac Progenitor Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NKX2.5 Antibody

Western Blot: NKX2.5 Antibody [NBP2-24675]

Western Blot: NKX2.5 Antibody [NBP2-24675]

Western Blot: Nkx2.5 Antibody [NBP2-24675] - Western blot analysis of Nkx2.5 in human heart lysate using 1) prebleed and 2) NBP2-24675 at 1 ug/ml.
Immunohistochemistry-Paraffin: NKX2.5 Antibody [NBP2-24675]

Immunohistochemistry-Paraffin: NKX2.5 Antibody [NBP2-24675]

Immunohistochemistry-Paraffin: Nkx2.5 Antibody [NBP2-24675] - Analysis of NKX2-5 in formalin-fixed, paraffin-embedded human brain tissue using an isotype control (top left) and NBP2-24675 (bottom left, right) at 5 ug/ml.

Applications for NKX2.5 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

5 ug/ml

Western Blot

1-2 ug/ml

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

0.2 ml PBS and 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NKX2.5

Homeobox-containing genes play critical roles in regulating tissue-specific gene expression essential for tissue differentiation, as well as determining the temporal and spatial patterns of development (Shiojima et al., 1995 [PubMed 7665173]). It has been demonstrated that a Drosophila homeobox-containing gene called 'tinman' is expressed in the developing dorsal vessel and in the equivalent of the vertebrate heart. Mutations in tinman result in loss of heart formation in the embryo, suggesting that tinman is essential for Drosophila heart formation. Furthermore, abundant expression of Csx, the presumptive mouse homolog of tinman, is observed only in the heart from the time of cardiac differentiation. CSX, the human homolog of murine Csx, has a homeodomain sequence identical to that of Csx and is expressed only in the heart, again suggesting that CSX plays an important role in human heart formation.[supplied by OMIM]

Long Name

NK2 Homeobox 5

Alternate Names

CSX1, NKX2-5, NKX2E

Gene Symbol

NKX2-5

UniProt

P52952

Additional NKX2.5 Products

Product Documents for NKX2.5 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for NKX2.5 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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