NLRP2/NALP2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-56154

Novus Biologicals, part of Bio-Techne
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NB100-56154
NB100-56154SS
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all NLRP2/NALP2 Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to amino acids 144-159 (LGKEVFKGKKPDKDNR) of human NALP2/PAN1/PYPAF2 was used as immunogen; GenBank no. gi|8923473|ref|NP_060322.1|.

Specificity

Performs similarly to the NALP2/PAN1/PYPAF2 which is cited in Bruey et al, 2004. The two antibodies are made against different portions of the NALP2 protein.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NLRP2/NALP2 Antibody

Western Blot: NLRP2/NALP2 Antibody [NB100-56154]

Western Blot: NLRP2/NALP2 Antibody [NB100-56154]

Western Blot: NLRP2/NALP2 Antibody [NB100-56154] - Analysis of NLRP2/NALP2 in differentiated THP-1 cells following IFN (interferon) stimulation using NALP2 antibody at 1:2000. TPA-differentiated THP-1 cells were cultured with 1000 units of IFNg for 0-24 hours as described in Bruey et al, 2004. Culturing differentiated THP-1 with IFNg resulted in transient elevations in NALP2 expression which peaked at 4-8 h after INFg treatment.
Immunohistochemistry-Paraffin: NLRP2/NALP2 Antibody [NB100-56154]

Immunohistochemistry-Paraffin: NLRP2/NALP2 Antibody [NB100-56154]

Immunohistochemistry-Paraffin: NLRP2/NALP2 Antibody [NB100-56154] - NLRP2 was detected in immersion fixed paraffin-embedded sections of human placenta using Rabbit Anti-Human NLRP2 polyclonal Antibody (Catalog # NB100-56154) at 1:1000 for 1 hour at room temperature followed by incubation with the Anti-Rabbit IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC003). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to the cytoplasm in horionic villi.

Applications for NLRP2/NALP2 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:1000-1:5000

Immunoprecipitation

1:50-1:200

Western Blot

1:1000-1:2000
Application Notes
Detected NALP2 at approx. 110 kDa in THP-1 cells.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

Whole antisera

Preservative

0.02% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NLRP2/NALP2

NALP2 (also known as PAN1/PYPAF2) is a member of the NLR (NACHT-LRR) family which includes both NODs and NALPs (Bruey et al, 2004). Alternative names for the NLR family include CATERPILLER, NOD and NOD-LRR. NLR family members are thought to be intracellular pathogen-recognition receptors (PRRs) (reviewed in Martionon and Tschopp, 2005 and 2007; Creagh and O'Neill, 2006; Petrilli et al, 2005). PRRs are key components of immune systems and are involved in innate effector mechanisms and activation of adaptive immunity. Toll-like receptors (TLRs) are the most well studied PPRs and overlaps have been identified between the signaling pathways used by some NLRs and TLRs suggesting redundancy and cooperation between NLR and TLR pathways. At least fourteen NALPs have been identified in the human genome, and a common feature among NALPs 1-3, 6, and 12 is their ability to to recruit the adaptor protein ASC through a homotypic PYD-PYD interaction, which in turn recruits caspase-1 through a CARD-CARD interaction. The oligomerization of NALPs is thought to bring the inflammatory caspases into close proximity leading to their activation within the inflammasome. The inflammasome is a multiprotein complex of more than 700 kDa that is responsible for the activation of caspases 1 and 5, which leads to the processing and secretion of the pro-inflammatory cytokines IL-1b and IL-18. The NALP2/3 inflammasomes contain, in addition to NALP2 or NALP3, the caspase recruitment domain (CARD)-containing protein Cardinal, ASC and caspase 1. Processing of the pro-inflammatory cytokines IL-1b and IL-18 by caspases leads to to the secretion of active cytokines and elicits a potent inflammatory response. Similar to TLRs, activation of the inflammasome by NLRs occurs through the recognition of pathogen-associated molecular patterns (PAMPs) through their LRR (leucine rich) domains): TLR family members recognize bacteria, viruses, fungi and protoza, whereas NLRs with known functions recognize bacteria. The expression of NALP2 is upregulated in THP-1 macrophage cells by LPS and certain cytokines including interferon g and b, supporting a role for NALP2 in host-defense and inflammation (Bruey et al, 2004). Additionally, NALP2 is expressed in several human tumor cell lines. However, the endogenous expression of NALP2 varies widely among tumor cells lines, with the highest levels found in MCF-7 and MDA-MB-435 breast cancer, Caco2 colon cancer and UACC62 melanoma cells (Bruey et al, 2004). This antibody recognizes NALP2; human NALP2 is a 1062 amino acid protein, it is specific for NALP2 does not cross-react with related family members PAN2 or NAC.

Long Name

NLR Family, Pyrin Domain Containing 2

Alternate Names

NALP2, NBS1, PAN1, PYPAF2

Entrez Gene IDs

55655 (Human)

Gene Symbol

NLRP2

Additional NLRP2/NALP2 Products

Product Documents for NLRP2/NALP2 Antibody

Product Datasheets

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Product Specific Notices for NLRP2/NALP2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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