NLRP3/NALP3 Antibody (25N10E9) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-03948

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Human, Mouse

Applications

Validated:

Simple Western, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Simple Western

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 25N10E9

Format

BSA Free

Concentration

1.0 mg/ml

View all NLRP3/NALP3 Antibodies »

Product Specifications

Immunogen

A recombinant protein corresponding to the NOD domain of murine NLRP3/NALP3 Antibody (25N10E9) (amino acids 216-385) was used as the immunogen for this antibody.

Specificity

The epitope that 25N10E9 binds to the NOD region of NLRP3/NALP3 has not been mapped.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for NLRP3/NALP3 Antibody (25N10E9) - BSA Free

Simple Western: NLRP3/NALP3 Antibody (25N10E9)BSA Free [NBP2-03948]

Simple Western: NLRP3/NALP3 Antibody (25N10E9)BSA Free [NBP2-03948]

Simple Western: NLRP3/NALP3 Antibody (25N10E9) [NBP2-03948] - Simple Western lane view shows a specific band for NALP3 in 0.5 mg/ml of RAW lysate. This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Western Blot: NLRP3/NALP3 Antibody (25N10E9)BSA Free [NBP2-03948]

Western Blot: NLRP3/NALP3 Antibody (25N10E9)BSA Free [NBP2-03948]

Western Blot: NLRP3/NALP3 Antibody (25N10E9) [NBP2-03948] - Analysis of Raw 264 cell lysate using NLRP3/NALP3 antibody (NBP2-03948) at 2 ug/ml.

Western Blot: NLRP3/NALP3 Antibody (25N10E9) [NBP2-03948]

Western Blot: NLRP3/NALP3 Antibody (25N10E9) [NBP2-03948] - Analysis using the NALP3 monoclonal antibody in mouse NALP3 transfected cell lysate (A), human NALP3 transfected cell lysate (B), mouse RAW lysate (C) and the recombinant mouse NLRP3 NOD domain protein immunogen (D) probed with 2 ug/ml antibody. Goat anti-mouse IgG HRP secondary antibody and PicoTect ECL substrate solution were used for this test.

Applications for NLRP3/NALP3 Antibody (25N10E9) - BSA Free

Application
Recommended Usage

Simple Western

1:50

Western Blot

2-5 ug/ml
Application Notes
In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NLRP3/NALP3

NACHT, LRR and PYD domains-containing protein 3 (NALP3), Nucleotide-Binding Oligomerization Domain, or Leucine Rich Repeat and Pyrin Domain Containing 3 (NLRP3) (human NLRP3-isoform 2 theoretical molecular weight 118kDa) acts as a cytosolic receptor for stress-signals initiated by a wide variety of pathogen-associated molecular patterns (PAMPS) and danger-associated molecular patterns (DAMPs) (1). NLRP3 belongs to the family of NOD-like receptors, a type of pattern-recognition receptor (PRR) which participates in innate immune responses. The exact mechanisms leading to NLRP3 activation are still not fully resolved. Some proposed mechanisms for NLRP3 inflammasome activation include induced ion channel flux as exemplified by potassium efflux, sodium influx and calcium signaling, generation of mitochondrial reactive oxygen species (ROS), and lysosomal destabilization (2). Activation of NLRP3 induces its association with the adaptor protein Apoptosis-associated Speck-like protein containing CARD (ASC) forming a complex that binds to Caspase-1 (1, 2). The inflammasome complex, comprised of NLRP3, ASC and Caspase 1, induces the processing of pro-IL-1beta and pro-IL-18 and release of the mature inflammatory cytokines IL-1beta and IL-18 and pyroptosis (1-3).

NLRP3 is expressed in a variety of cell types such as monocytes, dendritic cells, lymphocytes and epithelial cells (3). Abnormal NLRP3 activation may occur as the result of inherited mutations and is associated with diseases such as hereditary periodic fevers (HPFs) and familial cold autoinflammatory syndrome (FCAS). Additionally, abnormal NLRP3 activation is associated with a variety of diseases such as gout, obesity, atherosclerosis, Alzheimers, multiple sclerosis and type 2 diabetes (1, 3). NLRP3 inflammasome is regulated by several post-translational modifications (e.g., ubiquitination, phosphorylation and sumoylation) as well as by various interacting proteins (e.g., JNK1, FBXO3, TXNIP, MARK4 and PKD) (4).

References

1. Abderrazak, A., Syrovets, T., Couchie, D., El Hadri, K., Friguet, B., Simmet, T., & Rouis, M. (2015). NLRP3 inflammasome: From a danger signal sensor to a regulatory node of oxidative stress and inflammatory diseases. Redox Biology. https://doi.org/10.1016/j.redox.2015.01.008

2. Yang, Y., Wang, H., Kouadir, M., Song, H., & Shi, F. (2019). Recent advances in the mechanisms of NLRP3 inflammasome activation and its inhibitors. Cell Death and Disease. https://doi.org/10.1038/s41419-019-1413-8

3. Zahid, A., Li, B., Kombe, A. J. K., Jin, T., & Tao, J. (2019). Pharmacological inhibitors of the nlrp3 inflammasome. Frontiers in Immunology. https://doi.org/10.3389/fimmu.2019.02538

4. Kelley, N., Jeltema, D., Duan, Y., & He, Y. (2019). The NLRP3 inflammasome: An overview of mechanisms of activation and regulation. International Journal of Molecular Sciences. https://doi.org/10.3390/ijms20133328

Long Name

NLR Family, Pyrin Domain Containing 2

Alternate Names

AGTAVPRL, AII, CIAS1, Cryopyrin, FCAS, FCU, MWS, NACHT, NALP3, PYPAF1, NLRP3 monoclonal

Gene Symbol

NLRP3

UniProt

NP_001073289 (Human)

Additional NLRP3/NALP3 Products

Product Documents for NLRP3/NALP3 Antibody (25N10E9) - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for NLRP3/NALP3 Antibody (25N10E9) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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