NLRP3/NALP3 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-76806

Novus Biologicals, part of Bio-Techne
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NBP2-76806
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Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Peptide ELISA

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all NLRP3/NALP3 Antibodies »

Product Specifications

Immunogen

Peptide with sequence RDLYEKAKRDEPK-C corresponding to N-Terminus (near)of NLRP3/NALP3 Antibody according to NP_004886.3, NP_899632.1, NP_001120933.1, NP_001120934.1.

Epitope

RDLYEKAKRDEPK-C

Specificity

This antibody is expected to recognize all reported isoforms (NP_004886.3; NP_899632.1; NP_001120933.1; NP_001120934.1). Reported variants NP_001073289.1 and NP_004886.3 represent identical protein.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Theoretical MW

106 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NLRP3/NALP3 Antibody

Immunohistochemistry-Paraffin: NLRP3/NALP3 Antibody [NBP2-76806]

Immunohistochemistry-Paraffin: NLRP3/NALP3 Antibody [NBP2-76806]

Immunohistochemistry-Paraffin: NLRP3/NALP3 Antibody [NBP2-76806] - Staining of paraffin embedded Human peripheral blood leukocytes. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Applications for NLRP3/NALP3 Antibody

Application
Recommended Usage

Immunohistochemistry

5 ug/ml

Peptide ELISA

Detection Limit 1:32000
Application Notes
WB: Preliminary experiments gave an approx. 36 kDa band in human peripheral blood mononucleocytes lysates after 1 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the smallest calculated size of 106 kDa band according to NP_899632.1. The 36 kDa band was successfully blocked by incubation with the immunizing peptide.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 degrees C. Avoid freeze/thaw cycles.

Background: NLRP3/NALP3

NACHT, LRR and PYD domains-containing protein 3 (NALP3), Nucleotide-Binding Oligomerization Domain, or Leucine Rich Repeat and Pyrin Domain Containing 3 (NLRP3) (human NLRP3-isoform 2 theoretical molecular weight 118kDa) acts as a cytosolic receptor for stress-signals initiated by a wide variety of pathogen-associated molecular patterns (PAMPS) and danger-associated molecular patterns (DAMPs) (1). NLRP3 belongs to the family of NOD-like receptors, a type of pattern-recognition receptor (PRR) which participates in innate immune responses. The exact mechanisms leading to NLRP3 activation are still not fully resolved. Some proposed mechanisms for NLRP3 inflammasome activation include induced ion channel flux as exemplified by potassium efflux, sodium influx and calcium signaling, generation of mitochondrial reactive oxygen species (ROS), and lysosomal destabilization (2). Activation of NLRP3 induces its association with the adaptor protein Apoptosis-associated Speck-like protein containing CARD (ASC) forming a complex that binds to Caspase-1 (1, 2). The inflammasome complex, comprised of NLRP3, ASC and Caspase 1, induces the processing of pro-IL-1beta and pro-IL-18 and release of the mature inflammatory cytokines IL-1beta and IL-18 and pyroptosis (1-3).

NLRP3 is expressed in a variety of cell types such as monocytes, dendritic cells, lymphocytes and epithelial cells (3). Abnormal NLRP3 activation may occur as the result of inherited mutations and is associated with diseases such as hereditary periodic fevers (HPFs) and familial cold autoinflammatory syndrome (FCAS). Additionally, abnormal NLRP3 activation is associated with a variety of diseases such as gout, obesity, atherosclerosis, Alzheimers, multiple sclerosis and type 2 diabetes (1, 3). NLRP3 inflammasome is regulated by several post-translational modifications (e.g., ubiquitination, phosphorylation and sumoylation) as well as by various interacting proteins (e.g., JNK1, FBXO3, TXNIP, MARK4 and PKD) (4).

References

1. Abderrazak, A., Syrovets, T., Couchie, D., El Hadri, K., Friguet, B., Simmet, T., & Rouis, M. (2015). NLRP3 inflammasome: From a danger signal sensor to a regulatory node of oxidative stress and inflammatory diseases. Redox Biology. https://doi.org/10.1016/j.redox.2015.01.008

2. Yang, Y., Wang, H., Kouadir, M., Song, H., & Shi, F. (2019). Recent advances in the mechanisms of NLRP3 inflammasome activation and its inhibitors. Cell Death and Disease. https://doi.org/10.1038/s41419-019-1413-8

3. Zahid, A., Li, B., Kombe, A. J. K., Jin, T., & Tao, J. (2019). Pharmacological inhibitors of the nlrp3 inflammasome. Frontiers in Immunology. https://doi.org/10.3389/fimmu.2019.02538

4. Kelley, N., Jeltema, D., Duan, Y., & He, Y. (2019). The NLRP3 inflammasome: An overview of mechanisms of activation and regulation. International Journal of Molecular Sciences. https://doi.org/10.3390/ijms20133328

Long Name

NLR Family, Pyrin Domain Containing 2

Alternate Names

AGTAVPRL, AII, CIAS1, Cryopyrin, FCAS, FCU, MWS, NACHT, NALP3, PYPAF1

Entrez Gene IDs

114548 (Human)

Gene Symbol

NLRP3

UniProt

NP_004886.3, NP_899632.1 (Human), NP_001120933.1 (Human), NP_001120934.1 (Human)

Additional NLRP3/NALP3 Products

Product Documents for NLRP3/NALP3 Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for NLRP3/NALP3 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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