NLRX1 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-12566
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
Produced in rabbits immunized with E. coli-derived Human NLRX1 fragment
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Description
This antibody can be stored at 2-8C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20C to -80C. Avoid repeated freeze-thaw cycles.
Protein A & Antigen Affinity purified
Protein A & Antigen Affinity purified
Scientific Data Images for NLRX1 Antibody
Western Blot: NLRX1 Antibody [NBP3-12566]
Western Blot: NLRX1 Antibody [NBP3-12566] - Lane A: MCF7 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:108 kDaObserved band size:110 kDaImmunocytochemistry/ Immunofluorescence: NLRX1 Antibody [NBP3-12566]
Immunocytochemistry/Immunofluorescence: NLRX1 Antibody [NBP3-12566] - Staining of NLRX1 in A549 cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-Human NLRX1 polyclonal antibody (dilution ratio 1:200) at 4? overnight. Then cells were stained with the Alexa Fluor®488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.Immunohistochemistry-Paraffin: NLRX1 Antibody [NBP3-12566]
Immunohistochemistry-Paraffin: NLRX1 Antibody [NBP3-12566] - Staining of human NLRX1 in human urinary bladder with rabbit polyclonal antibody at 1:100 dilution.Applications for NLRX1 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:500
Immunohistochemistry-Paraffin
1:50-1:200
Immunoprecipitation
1-5uL/mg of uLysate
Western Blot
1:500-1:2000
Formulation, Preparation, and Storage
Purification
Antigen and protein A Affinity-purified
Formulation
PBS
Preservative
0.03% Proclin 300
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: NLRX1
Alternate Names
Caterpiller protein 11.3, CLR11.3DLNB26, MGC131937, MGC21025, NLR family member X1, NLR family, X1, NOD26, NOD5, NOD9FLJ21478, NOD-like receptor X1, Nucleotide-binding oligomerization domain protein 26, Nucleotide-binding oligomerization domain protein 5, Nucleotide-binding oligomerization domain protein 9, nucleotide-binding oligomerization domain, leucine rich repeat containing X1
Gene Symbol
NLRX1
Additional NLRX1 Products
Product Specific Notices for NLRX1 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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