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NM23-H2/NME2 Antibody (2E3) - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-60241

Novus Biologicals, part of Bio-Techne
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NBP2-60241-100ul
NBP2-60241-50ul

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2E3

Format

BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

Recombinant human NM23-H2/NME2 (1-152aa) purified from E. coli

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for NM23-H2/NME2 Antibody (2E3) - BSA Free

Western Blot: NM23-H2/NME2 Antibody (2E3)BSA Free [NBP2-60241]

Western Blot: NM23-H2/NME2 Antibody (2E3)BSA Free [NBP2-60241]

Western Blot: NM23-H2/NME2 Antibody (2E3) [NBP2-60241] - The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NM23-H2/NME2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: HeLa cell lysateLane 2.: A549 cell lysate Lane 3.: MCF7 cell lysate Lane 4.: LnCap cell lysateThe cell lysates (5ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human NME2 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: 293T cell lysateLane 2.: NME2 transfected 293T cell lysate
Immunocytochemistry/ Immunofluorescence: NM23-H2/NME2 Antibody (2E3) - BSA Free [NBP2-60241]

Immunocytochemistry/ Immunofluorescence: NM23-H2/NME2 Antibody (2E3) - BSA Free [NBP2-60241]

Immunocytochemistry/Immunofluorescence: NM23-H2/NME2 Antibody (2E3) [NBP2-60241] - Staining in HeLa cells. The secondary antibody (green) was Alexa Fluor 488. DAPI was stained the cell nucleus (blue).

Applications for NM23-H2/NME2 Antibody (2E3) - BSA Free

Application
Recommended Usage

ELISA

1:100 - 1:2000

Immunocytochemistry/ Immunofluorescence

1:100

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.4), 10% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NM23-H2

Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by NME1) and 'B' (encoded by this gene) isoforms. Multiple alternatively spliced transcript variants encoding the same isoform have been found for this gene. Co-transcription of this gene and the neighboring upstream gene (NME1) generates naturally-occurring transcripts (NME1-NME2) which encode a fusion protein comprised of sequence sharing identity with each individual gene product.

Long Name

Non-metastatic Protein 23 Homolog 2

Alternate Names

NDK B, NDP Kinase B2, NDPKB, NM23B, NME2

Gene Symbol

NME2

Additional NM23-H2 Products

Product Documents for NM23-H2/NME2 Antibody (2E3) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for NM23-H2/NME2 Antibody (2E3) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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