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NM23-H2/NME2 Antibody (NME2/6435)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-20542

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # NME2/6435

Concentration

0.2 mg/ml

Product Specifications

Immunogen

Recombinant full-length human NM23-H2/NME2 protein

Localization

Cytoplasm. Nucleus.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Positive Controls: PC3, A549, HeLa or Jurkat cells. Ubiquitously expressed in all tissues.

Antibody with azide - store at 2 to 8C. Antibody without azide - store at -20 to -80C. Non-hazardous. No MSDS required.

Scientific Data Images for NM23-H2/NME2 Antibody (NME2/6435)

NM23-H2/NME2 Antibody (NME2/6435)

NM23-H2/NME2 Antibody (NME2/6435)

Analysis of Protein Array containing more than 19,000 full-length human proteins using NM23-H2/NME2 Monoclonal Antibody (NME2/6435).
NM23-H2/NME2 Antibody (NME2/6435)

NM23-H2/NME2 Antibody (NME2/6435)

Formalin-fixed, paraffin-embedded human breast carcinoma stained with NM23-H2/NME2 antibody (NME2/6435). HIER: Tris/EDTA, pH9.0, 45min. Secondary: HRP-polymer, 30min. DAB, 5min.

Applications for NM23-H2/NME2 Antibody (NME2/6435)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1-2 ug/ml
Application Notes
Immunohistochemistry (Formalin-fixed): 1-2ug/ml for 30 minutes. at RT. Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95C followed by cooling at RT for 20 minutes.

Optimal dilution for a specific application should be determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM PBS with 0.05% BSA

Preservative

0.05% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: NM23-H2

Nucleoside diphosphate kinase (NDK) exists as a hexamer composed of 'A' (encoded by NME1) and 'B' (encoded by this gene) isoforms. Multiple alternatively spliced transcript variants encoding the same isoform have been found for this gene. Co-transcription of this gene and the neighboring upstream gene (NME1) generates naturally-occurring transcripts (NME1-NME2) which encode a fusion protein comprised of sequence sharing identity with each individual gene product.

Long Name

Non-metastatic Protein 23 Homolog 2

Alternate Names

NDK B, NDP Kinase B2, NDPKB, NM23B, NME2

Gene Symbol

NME2

Additional NM23-H2 Products

Product Documents for NM23-H2/NME2 Antibody (NME2/6435)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for NM23-H2/NME2 Antibody (NME2/6435)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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