NMDAR2B Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NB100-41097
Key Product Details
Species Reactivity
Validated:
Human, Rat
Cited:
Human, Rat
Predicted:
Canine (100%), Mouse (100%). Backed by our 100% Guarantee.
Applications
Validated:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Peptide ELISA, Western Blot
Cited:
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Frozen
Label
Unconjugated
Antibody Source
Polyclonal Goat IgG
Concentration
0.5 mg/ml
Product Specifications
Immunogen
Peptide with sequence C-KDAHEKDDFHHLS corresponding to internal region according to NP_000825.1.
Localization
Cytoskeleton/cytoplasm
Clonality
Polyclonal
Host
Goat
Isotype
IgG
Scientific Data Images for NMDAR2B Antibody
![Western Blot: NMDAR2B Antibody [NB100-41097]](https://resources.bio-techne.com/images/products/NMDAR2B-Antibody-Western-Blot-NB100-41097-img0003.jpg "Western Blot: NMDAR2B Antibody [NB100-41097]")
Western Blot: NMDAR2B Antibody [NB100-41097]
Western Blot: NMDAR2B Antibody [NB100-41097] - (2ug/ml) staining of Rat Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.![Immunocytochemistry/ Immunofluorescence: NMDAR2B Antibody [NB100-41097]](https://resources.bio-techne.com/images/products/NMDAR2B-Antibody-Immunocytochemistry-Immunofluorescence-NB100-41097-img0004.jpg "Immunocytochemistry/ Immunofluorescence: NMDAR2B Antibody [NB100-41097]")
Immunocytochemistry/ Immunofluorescence: NMDAR2B Antibody [NB100-41097]
Immunocytochemistry/Immunofluorescence: NMDAR2B Antibody [NB100-41097] - Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing cytoskeleton and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).Applications for NMDAR2B Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
10 ug/ml
Peptide ELISA
Detection limit 1:16000
Western Blot
2 - 4 ug/ml
Application Notes
WB: Approx. 170 kDa band observed in rat brain lysates (calculated MW of 166 kDa band according to NP_000825.1). Use in Immunohistochemistry-Frozen reported in scientific literature (PMID 28206989).
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA
Preservative
0.02% Sodium Azide
Concentration
0.5 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: NMDAR2B
Alternate Names
glutamate [NMDA] receptor subunit epsilon-2, glutamate receptor subunit epsilon-2, glutamate receptor, ionotropic, N-methyl D-aspartate 2B, hNR3, MGC142180, NMDAR2BMGC142178, N-methyl D-aspartate receptor subtype 2B, N-methyl-D-aspartate receptor subunit 3, NR2B, NR3
Gene Symbol
GRIN2B
UniProt
Additional NMDAR2B Products
Product Documents for NMDAR2B Antibody
Product Specific Notices for NMDAR2B Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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