NMDAR2B [p Tyr1472] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB300-182

Novus Biologicals, part of Bio-Techne
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NB300-182
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Human, Rat

Predicted:

Bovine (100%), Canine (100%), Chicken (100%), Primate (100%), Zebrafish (100%). Backed by our 100% Guarantee.

Applications

Validated:

Simple Western, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all NMDAR2B Antibodies »

Product Specifications

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Tyr1472 conjugated to KLH. Accession # Q00960

Reactivity Notes

Human reactivity reported in scientific literature (PMID: 28273943).

Modification

p Tyr1472

Localization

Integral membrane protein.

Specificity

Specific for endogenous levels of the ~180 kDa NMDAR2B protein phosphorylated at Tyr1472. Immunolabeling is completely eliminated by treatment with lambda-phosphatase.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

180 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NMDAR2B [p Tyr1472] Antibody

Western Blot: NMDAR2B [p Tyr1472] Antibody [NB300-182]

Western Blot: NMDAR2B [p Tyr1472] Antibody [NB300-182]

Western Blot: NMDAR2B [p Tyr1472] Antibody [NB300-182] - Rat hippocampal lysate showing specific immunolabeling of the ~180 kDa NR2B subunit of the NMDAR phosphorylated at Tyr1472 in the first lane (-). Phosphospecificity is shown in the second lane (+) where immunolabeling is completely eliminated by lysate treatment with lambda phosphatase (400 units/100uL lysate for 30 min).

Applications for NMDAR2B [p Tyr1472] Antibody

Application
Recommended Usage

Western Blot

1:1000
Application Notes
NOTE: Immunolabeling is blocked by the phosphopeptide but not by the dephosphopeptide. The antibody also labels bands at ~65kDa and ~115kDa. See Simple Western Antibody Database for Simple Western validation: Tested in mouse brain lysates; separated by size; antibody dilution of 1:10.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

10 mM HEPES (pH 7.5), 0.15 M NaCl, 0.1 mg/mL BSA, 50% Glycerol

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NMDAR2B

The ion channels activated by glutamate are typically divided into two classes. Those that are sensitive to N-methyl-D-aspartate (NMDA) are designated NMDA receptors (NMDAR). The NMDAR plays an essential role in memory, neuronal development and it has also been implicated in several disorders of the central nervous system including Alzheimer's, epilepsy and ischemic neuronal cell death. Overexpression of the NR2B subunit of the receptor has been associated with increases in learning and memory while aged, memory impaired animals have deficiencies in NR2B expression. Tyr1472 on NR2B is phosphorylated and this may lead to the increased expression of the NMDAR at the synapse that plays a role in synaptic plasticity.

Alternate Names

glutamate [NMDA] receptor subunit epsilon-2, glutamate receptor subunit epsilon-2, glutamate receptor, ionotropic, N-methyl D-aspartate 2B, hNR3, MGC142180, NMDAR2BMGC142178, N-methyl D-aspartate receptor subtype 2B, N-methyl-D-aspartate receptor subunit 3, NR2B, NR3

Entrez Gene IDs

24410 (Rat)

Gene Symbol

GRIN2B

UniProt

Q00960 (Rat)

Additional NMDAR2B Products

Product Documents for NMDAR2B [p Tyr1472] Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for NMDAR2B [p Tyr1472] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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