NMDAR2B [p Tyr1252] Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB300-292

Novus Biologicals, part of Bio-Techne
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NB300-292
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Mouse, Rat

Predicted:

Bovine (100%), Canine (100%), Chicken (100%), Human (100%), Primate (100%), Zebrafish (100%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all NMDAR2B Antibodies »

Product Specifications

Immunogen

Synthetic phospho-peptide corresponding to amino acid residues surrounding Tyr1252 conjugated to KLH. Accession # Q00960

Modification

p Tyr1252

Localization

Integral membrane protein.

Specificity

Specific for endogenous levels of the ~180 kDa NMDAR2B protein phosphorylated at Tyr1252 . Immunolabeling is completely eliminated by treatment with lambda-phosphatase. May also show slight reactivity with Tyr1246 of NR2A.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

180 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NMDAR2B [p Tyr1252] Antibody

Western Blot: NMDAR2B [p Tyr1252] Antibody [NB300-292]

Western Blot: NMDAR2B [p Tyr1252] Antibody [NB300-292]

Western Blot: NMDAR2B [p Tyr1252] Antibody [NB300-292] - Rat hippocampal lysate showing specific immunolabeling of the ~180k NR2B subunit phosphorylated at Tyr1252 (Control). Phosphospecificity is shown in the second lane (lambda-phosphatase: lambda-Ptase). The blot is identical to the control except that it was incubated in lambda-Ptase (1200 units for 30 min) before being exposed to the phospho-Tyr1252 NMDA NR2B subunit antibody. The immunolabeling is completely eliminated by treatment with lambda-Ptase.
Immunohistochemistry: NMDAR2B [p Tyr1252] Antibody [NB300-292]

Immunohistochemistry: NMDAR2B [p Tyr1252] Antibody [NB300-292]

Immunohistochemistry: NMDAR2B [p Tyr1252] Antibody [NB300-292] - Immunostaining of 14 DIV rat cortical neurons showing NR2B phosphorylated at Tyr1252 in red and PSD95 in green. Photo courtesy of Gang Liu

Applications for NMDAR2B [p Tyr1252] Antibody

Application
Recommended Usage

Immunohistochemistry

1:400

Western Blot

1:1000
Application Notes
This antibody has been tested in Western blot on rat brain hippocampal lysate, detecting a band 180 kDa.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NMDAR2B

The NMDA receptor (NMDAR) plays an essential role in memory, neuronal development and it has been implicated in several disorders of the CNS including Alzheimer's, epilepsy and ischemic neuronal death. Phosphorylation of Tyr 1252 is thought to potentiate NMDAR dependent influx of calcium. (1-6)

Alternate Names

glutamate [NMDA] receptor subunit epsilon-2, glutamate receptor subunit epsilon-2, glutamate receptor, ionotropic, N-methyl D-aspartate 2B, hNR3, MGC142180, NMDAR2BMGC142178, N-methyl D-aspartate receptor subtype 2B, N-methyl-D-aspartate receptor subunit 3, NR2B, NR3

Entrez Gene IDs

2904 (Human); 24410 (Rat)

Gene Symbol

GRIN2B

UniProt

Q00960 (Rat)

Additional NMDAR2B Products

Product Documents for NMDAR2B [p Tyr1252] Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for NMDAR2B [p Tyr1252] Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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