NMDAR2B Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-30037

Novus Biologicals, part of Bio-Techne
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NBP1-30037
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Key Product Details

Species Reactivity

Validated:

Mouse, Rat

Predicted:

Bovine (100%), Canine (100%), Chicken (100%), Human (100%), Mouse (100%), Primate (100%). Backed by our 100% Guarantee.

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all NMDAR2B Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to amino acid residues from the N-terminal region of the NMDAR2B subunit conjugated to KLH. Accession # Q00960

Reactivity Notes

It is anticipated that the antibody will also react with bovine, canine, chicken, human, mouse and non-human primate based on the fact that these species have 100 % homology with the amino acid sequence used as antigen.

Specificity

Specific for endogenous levels of the ~180 kDa NR2B subunit of the NMDA receptor.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

180 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NMDAR2B Antibody

Western Blot: NMDAR2B Antibody [NBP1-30037]

Western Blot: NMDAR2B Antibody [NBP1-30037]

Western Blot: NMDAR2B Antibody [NBP1-30037]
Immunohistochemistry: NMDAR2B Antibody [NBP1-30037]

Immunohistochemistry: NMDAR2B Antibody [NBP1-30037]

Immunohistochemistry: NMDAR2B Antibody [NBP1-30037] - Immunostaining of 14 DIV rat cortical neurons showing NR2B in green and PSD95 in red.

Applications for NMDAR2B Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:400

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:500

Immunohistochemistry-Paraffin

1:500

Western Blot

1:1000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

10mM HEPES (pH 7.5), 0.15M NaCl, 0.1 mg/ml BSA and 50% Glycerol

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NMDAR2B

N-methyl-D-aspartate (NMDA) receptors are ligand-gated ion channels that have a high permeability to calcium found in the central nervous system. The receptor consists of a number of distinct ligand binding domains, and the presence of both glutamate and glycine are required for full activation of the channel. Within the channel there is also a binding site for magnesium, which, when occupied, propagates a voltage-dependent channel block. Other binding sites are also found in the receptor, including a zinc-binding site and an inter-channel site that binds specific channel blockers such as phencyclidine (PCP) and related compounds. The NMDA receptor has been demonstrated to play an essential role in long-term potentiation (LTP), a phenomenon that has been implicated to be the basis for learning and memory. The influx of calcium as a result of channel activation is thought to be responsible for neuronal plasticity and glutamate neurotoxicity. A number of different NMDA receptor subunits have been cloned that may possess different functional and localization properties. The NMDA-R1 subunit (NR1) is expressed throughout the brain, while the NMDA-R2 subunits (NR2A, NR2B, NR2C, and NR2D) have a more specific localization pattern. The NMDA receptor subunits differ also in glycine sensitivity, the relative strength of the magnesium channel block, and their respective agonist-dependent deactivation time. Differential splicing of three distinct exons of NR1 generates eight NR1 splice variants. These exons encode a 21 amino acid N-terminal domain (N1) and two 21 amino acid C-terminal domains (C1 and C2, respectively). Splicing out the C2 cassette eliminates a stop codon, generating a new 22 amino acid C-terminal domain (C2'). These splice variants have differing patterns of expression.

Alternate Names

glutamate [NMDA] receptor subunit epsilon-2, glutamate receptor subunit epsilon-2, glutamate receptor, ionotropic, N-methyl D-aspartate 2B, hNR3, MGC142180, NMDAR2BMGC142178, N-methyl D-aspartate receptor subtype 2B, N-methyl-D-aspartate receptor subunit 3, NR2B, NR3

Entrez Gene IDs

2904 (Human); 14812 (Mouse); 24410 (Rat); 494009 (Canine)

Gene Symbol

GRIN2B

UniProt

Q00960 (Rat)

Additional NMDAR2B Products

Product Documents for NMDAR2B Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for NMDAR2B Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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