NMT2 Antibody (2E12-4B5)

Novus Biologicals, part of Bio-Techne | Catalog # H00009397-M01

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 2E12-4B5

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all NMT2 Antibodies »

Product Specifications

Immunogen

NMT2 (AAH06376, 1 a.a. ~ 498 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MAEDSESAASQQSLELDDQDTCGIDGDNEEETEHAKGSPGGYLGAKKKKKKQKRKKEKPNSGGTKSDSASDSQEIKIQQPSKNPSVPMQKLQDIQRAMELLSACQGPARNIDEAAKHRYQFWDTQPVPKLDEVITSHGAIEPDKDNVRQEPYSLPQGFMWDTLDLSDAEVLKELYTLLNENYVEDDDNMFRFDYSPEFLLWALRPPGWLLQWHCGVRVSSNKKLVGFISAIPANIRIYDSVKKMVEINFLCVHKKLRSKRVAPVLIREITRRVNLEGIFQAVYTAGVVLPKPIATCRYWHRSLNPKKLVEVKFSHLSRNMTLQRTMKLYRLPDVTKTSGLRPMEPKDIKSVRELINTYLKQFHLAPVMDEEEVAHWFLPREHIIDTFVVESPNGKLTDFLSFYTLPSTVMHHPAHKSLKAAYSFYNIHTETPLLDLMSDALILAKSKGFDVFNALDLMENKTFLEKLKFGIGDGNLQYYLYNWRCPGTDSEKVGLVLQ

Reactivity Notes

Human. Other species not tested.

Specificity

NMT2 - N-myristoyltransferase 2

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Scientific Data Images for NMT2 Antibody (2E12-4B5)

Western Blot: NMT2 Antibody (2E12-4B5) [H00009397-M01]

Western Blot: NMT2 Antibody (2E12-4B5) [H00009397-M01]

Western Blot: NMT2 Antibody (2E12-4B5) [H00009397-M01] - Analysis of NMT2 expression in transfected 293T cell line by NMT2 monoclonal antibody (M01), clone S1.Lane 1: NMT2 transfected lysate (Predicted MW: 57 KDa).Lane 2: Non-transfected lysate.

Applications for NMT2 Antibody (2E12-4B5)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody Reactive against Recombinant Protein with GST tag on ELISA and Western Blot. GST tag alone is used as a negative control.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: NMT2

N-myristoyltransferase (NMT) catalyzes the reaction of N-terminal myristoylation of many signaling proteins. It transfers myristic acid from myristoyl coenzyme A to the amino group of a protein's N-terminal glycine residue. Biochemical evidence indicates the presence of several distinct NMTs, varying in apparent molecular weight and /or subcellular distribution. The predicted 498-amino acid of human NMT2 protein shares 77% and 96% sequence identity with human NMT1 and mouse Nmt2 comprise two distinct families of N-myristoyltransferases.

Alternate Names

EC 2.3.1.97, glycylpeptide N-tetradecanoyltransferase 2, Myristoyl-CoA:protein N-myristoyltransferase 2, NMT 2, N-myristoyltransferase 2, Peptide N-myristoyltransferase 2, Type II N-myristoyltransferase

Entrez Gene IDs

9397 (Human)

Gene Symbol

NMT2

OMIM

603801 (Human)

UniProt

AAH06376 (Human)

Additional NMT2 Products

Product Documents for NMT2 Antibody (2E12-4B5)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for NMT2 Antibody (2E12-4B5)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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