nNOS Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-97555

Novus Biologicals, part of Bio-Techne
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NBP1-97555-0.025mg
NBP1-97555-0.05mg
NBP1-97555-0.2mg
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Key Product Details

Species Reactivity

Validated:

Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all nNOS Antibodies »

Product Summary for nNOS Antibody

Immunogen

Synthetic peptide corresponding to the sequence near the C-terminus of human nNOS.

Reactivity Notes

Recognizes Mouse and Rat nNOS.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for nNOS Antibody

Western Blot: nNOS Antibody [NBP1-97555]

Western Blot: nNOS Antibody [NBP1-97555]

Western Blot: nNOS Antibody [NBP1-97555] - Analysis: Lane 1: MW marker, Lane 2: Rat Brain, Lane 3: Mouse Brain.
Immunohistochemistry-Paraffin: nNOS Antibody [NBP1-97555]

Immunohistochemistry-Paraffin: nNOS Antibody [NBP1-97555]

Immunohistochemistry-Paraffin: nNOS Antibody [NBP1-97555] - Human brain, cortex tissue. Dilution at 20ug/ml.

Applications for nNOS Antibody

Application
Recommended Usage

Immunohistochemistry

20 ug/ml

Immunohistochemistry-Paraffin

20 ug/ml

Western Blot

1:250
Application Notes
This antibody is useful in Western Blot (Detects a band of ~155kDa).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 50% Glycerol

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: nNOS

The diffusible free radical gas nitric oxide (NO) affects a variety of physiological functions, and is a key regulator of the cardiovascular, nervous, and immune systems. NO is synthesized in many tissues from L-arginine, oxygen, and NADPH by three known isoforms of a heme-containing flavoprotein termed NO synthase (nNOS/NOS-I, iNOS/NOS-II, and eNOS/NOS-III). nNOS is a constitutively expressed neuronal NOS isoform that exits in its latent form until it is activated by the binding of calmodulin follo wing elevation of intracellular calcium levels. The C-terminus of nNOS contains a conserved serine residue, Ser1417, analogous to Ser1177 of the constitutively expressed endothelial NOS isoform (eNOS). Phosphorylation of Ser1417 is believed to regulate nNOS activation, particularly in glucose-sensing neurons, where inhibition of AMPK pathways by glucose and leptin serve to suppress nNOS activity, whereas activation of AMPK by insulin leads to nNOS activation.

Long Name

Neuronal Nitric Oxide Synthase

Alternate Names

NOS1

Gene Symbol

NOS1

Additional nNOS Products

Product Documents for nNOS Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for nNOS Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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