NNT Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-86512

Novus Biologicals, part of Bio-Techne
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NBP1-86512
NBP1-86512-25ul
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all NNT Antibodies »

Product Summary for NNT Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: ANLLKTVVTGCSCPLLSNLGSCKGLRVKKDFLRTFYTHQELWCKAPVKPGIPYKQLTVGVPKEIFQNEKRVALSPAGVQNLVKQGFNVVVESGAGEASKFSDDHYRVAGAQIQGAKEVLASDLVVKVRAPMVNPTLGVHEADLLKTSGT

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (86%), Rat (87%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NNT Antibody

Western Blot: NNT Antibody [NBP1-86512]

Western Blot: NNT Antibody [NBP1-86512]

Western Blot: NNT Antibody [NBP1-86512] - Analysis in human cell lines HeLa and U-251MG. Corresponding RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.
Immunohistochemistry-Paraffin: NNT Antibody [NBP1-86512]

Immunohistochemistry-Paraffin: NNT Antibody [NBP1-86512]

Immunohistochemistry-Paraffin: NNT Antibody [NBP1-86512] - Staining in human heart muscle and skin tissues using NBP1-86512 antibody. Corresponding NNT RNA-seq data are presented for the same tissues.
Immunohistochemistry-Paraffin: NNT Antibody [NBP1-86512]

Immunohistochemistry-Paraffin: NNT Antibody [NBP1-86512]

Immunohistochemistry-Paraffin: NNT Antibody [NBP1-86512] - Staining of human heart muscle shows strong granular cytoplasmic positivity in cardiomyocytes.
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Applications for NNT Antibody

Application
Recommended Usage

Immunohistochemistry

1:200 - 1:500

Immunohistochemistry-Paraffin

1:200 - 1:500

Western Blot

0.04 - 0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NNT

NNT encodes an integral protein of the inner mitochondrial membrane. The enzyme couples hydride transfer between NAD(H) and NADP(+) to proton translocation across the inner mitochondrial membrane. Under most physiological conditions, the enzyme uses energy from the mitochondrial proton gradient to produce high concentrations of NADPH. The resulting NADPH is used for biosynthesis and in free radical detoxification. Two alternatively spliced variants, encoding the same protein, have been found for this gene.

Alternate Names

MGC126502, mitochondrial, nicotinamide nucleotide transhydrogenaseMGC126503, Pyridine nucleotide transhydrogenase

Gene Symbol

NNT

Additional NNT Products

Product Documents for NNT Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for NNT Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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