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Nogo-A Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-41070

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

Product Specifications

Immunogen

Antibody was raised against a 23 amino acid synthetic peptide from near the amino terminus of human NogoA. The immunogen is located within the first 50 amino acids of NogoA. Amino Acid Squence: SDSPPRPQPAFKYQFVREPEDEE

Specificity

At least five isoforms of Nogo are known to exist; this antibody is specific for NogoA.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Nogo-A Antibody - BSA Free

Western Blot: Nogo-A AntibodyBSA Free [NBP2-41070]

Western Blot: Nogo-A AntibodyBSA Free [NBP2-41070]

Western Blot: Nogo-A Antibody [NBP2-41070] - Western blot analysis of NogoA in mouse brain tissue lysate with NogoA antibody at (A) 0.5 and (B) 1 ug/mL.
Immunocytochemistry/ Immunofluorescence: Nogo-A Antibody - BSA Free [NBP2-41070]

Immunocytochemistry/ Immunofluorescence: Nogo-A Antibody - BSA Free [NBP2-41070]

Immunocytochemistry/Immunofluorescence: Nogo-A Antibody [NBP2-41070] - Immunofluorescence of NogoA in Mouse Brain cells with NogoA antibody at 20 ug/mL.
Immunohistochemistry-Paraffin: Nogo-A Antibody - BSA Free [NBP2-41070]

Immunohistochemistry-Paraffin: Nogo-A Antibody - BSA Free [NBP2-41070]

Immunohistochemistry-Paraffin: Nogo-A Antibody [NBP2-41070] - Immunohistochemistry of NogoA in mouse brain tissue with NogoA antibody at 2.5 ug/mL.

Applications for Nogo-A Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

20 ug/ml

Immunohistochemistry

2.5 ug/ml

Immunohistochemistry-Paraffin

2.5 ug/ml

Western Blot

0.5-1 ug/ml
Application Notes
Despite its predicted molecular weight, Nogo-A typically migrates at 180 kDa in an SDS-PAGE.

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Nogo-A

Nogo-A is a member of the reticulon family of transmembrane proteins. This family is characterized by the presence of a nonsignal sequence containing N-terminus, a topologically conserved 200 amino acid C-terminus that contains two transmembrane domains, and an ER-retention motif. Nogo-A has punctate intracellular distribution within the ER that is reminescent of a reticulum.

Long Name

Reticulon 4A

Alternate Names

NI220, NogoA, RTN4, RTN4A

Gene Symbol

RTN4

Additional Nogo-A Products

Product Documents for Nogo-A Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Nogo-A Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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