Nogo Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB100-56681

Novus Biologicals, part of Bio-Techne
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NB100-56681SS
NB100-56681
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Rabbit, Sheep

Cited:

Human, Mouse, Rat, Ovine, Rabbit

Applications

Validated:

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot

Cited:

ELISA, IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all Nogo Antibodies »

Product Specifications

Immunogen

A synthetic peptide to an internal portion of human Nogo A (between amino acids 1-50) [UniProt Q9NQC3].

Reactivity Notes

Rabbit reactivity reported in scientific literature (PMID: 24223763).

Specificity

This recognizes Nogo-A (approx. 220 kD)and Nogo-B2 and B1 (approx. 50 kD and 48 kD in human and 43 kD and 41 kD in mouse and rat, respectively).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Nogo Antibody

Western Blot: Nogo Antibody [NB100-56681]

Western Blot: Nogo Antibody [NB100-56681]

Western Blot: Nogo Antibody [NB100-56681] - Analysis of Nogo A and B in A) human, B) mouse, and C) rat brain tissue lysate using Nogo antibody at 1:2000.
Immunocytochemistry/ Immunofluorescence: Nogo Antibody [NB100-56681]

Immunocytochemistry/ Immunofluorescence: Nogo Antibody [NB100-56681]

Immunocytochemistry/Immunofluorescence: Nogo Antibody [NB100-56681] - Increased lipid droplet biogenesis buffers lack spastin-M1 at the ER. MEF cell lines were starved in HBSS and treated either with inhibitors of DGAT1 and DGAT2 (D1+D2i) or DMSO overnight and stained with antibodies against Nogo antibody. Representative single-plane confocal images. Scale bar: 10 um. Image collected and cropped by CiteAb from the following publication (https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7184029/) licensed under a CC-BY license.
Immunohistochemistry-Paraffin: Nogo Antibody [NB100-56681]

Immunohistochemistry-Paraffin: Nogo Antibody [NB100-56681]

Immunohistochemistry-Paraffin: Nogo Antibody [NB100-56681] - Analysis of human testis using Nogo antibody at 1:100.
View 1 more images

Applications for Nogo Antibody

Application
Recommended Usage

ELISA

reported in scientific literature (PMID 17413036)

Immunocytochemistry/ Immunofluorescence

reported in scientific literature (PMID 27132996)

Immunohistochemistry

reported in scientific literature (PMID 30208932)

Immunohistochemistry-Paraffin

1:100

Simple Western

1:1000

Western Blot

1:500-1:2000
Application Notes
This antibody recognizes Nogo A (approximately 220 kD) and Nogo B2 and B1 (approximately 50 kD and 48 kD in human and 43 kD and 41 kD in mouse and rat, respectively).

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point. Separated by Size-Wes, Sally Sue/Peggy Sue.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Nogo

Nogo-B is a novel myelin-associated, intermediate-length splice variant of Nogo belonging to the reticulon family. It is a multidomain neurite growth inhibitory protein containing several discrete regions with growth inhibitory functions and is a component of the CNS myelin that prevents axonal regeneration in the adult vertebrate CNS. Nogo-B along with NGBR mediated chemotaxis in HUVECs and induced tube formation in 3-dimensional cultures. It is mostly found in intact blood vessels, smooth muscle cells, and endothelial cell, with its N terminus oriented extracellularly and is a regulator of vascular homeostasis and remodeling. It plays an important role in sporadic and familial amyotrophic lateral sclerosis

Alternate Names

ASY, foocen, Human NogoA, KIAA0886, My043 protein, Nbla00271, Nbla10545, neurite growth inhibitor 220, Neurite outgrowth inhibitor, Neuroendocrine-specific protein, Neuroendocrine-specific protein C homolog, NI220/250, Nogo protein, NOGO-A, Nogo-B, NOGOC, Nogo-C, NOGORTN4-B1, NSP, NSP-CL, reticulon 4, reticulon 5, reticulon-4, Reticulon-5, RTN4-A, RTN4-B2, RTN4-C, RTN-x

Entrez Gene IDs

57142 (Human); 68585 (Mouse); 83765 (Rat)

Gene Symbol

RTN4

UniProt

Q9NQC3

Additional Nogo Products

Product Documents for Nogo Antibody

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Nogo Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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