Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all NOL12 Antibodies »

Product Specifications

Immunogen

The immunogen for this product maps to a region between residue 163 and 213 of human Nucleolar Protein 12 using the numbering given in entry NP_077289.1 (GeneID 79159).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NOL12 Antibody

Western Blot: NOL12 Antibody [NBP1-46174]

Western Blot: NOL12 Antibody [NBP1-46174]

Western Blot: NOL12 Antibody [NBP1-46174] - Detection of human and mouse NOL12 by western mlot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NOL12 antibody NBP1-46174 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunohistochemistry: NOL12 Antibody [NBP1-46174]

Immunohistochemistry: NOL12 Antibody [NBP1-46174]

Immunohistochemistry: NOL12 Antibody [NBP1-46174] - Detection of human NOL12 by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-NOL12 (NBP1-46174) Detection: DAB
Western Blot: NOL12 Antibody [NBP1-46174]

Western Blot: NOL12 Antibody [NBP1-46174]

Western Blot: NOL12 Antibody [NBP1-46174] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-NOL12 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. NOL12 was also immunoprecipitated by rabbit anti-NOL12 antibody NBP1-46173, which recognizes an upstream epitope.
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Applications for NOL12 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:10-1:500

Immunoprecipitation

2-5 ug/mg lysate

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: NOL12

NOL12 may bind to 28S rRNA

Alternate Names

dJ37E16.7, FLJ34609, MGC3731, Nop25, nucleolar protein 12, RRP17

Gene Symbol

NOL12

Additional NOL12 Products

Product Documents for NOL12 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for NOL12 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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