NOL12 Antibody - BSA Free
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-46174
![Immunohistochemistry: NOL12 Antibody [NBP1-46174]](https://resources.bio-techne.com/images/products/NOL12-Antibody-Immunohistochemistry-NBP1-46174-img0003.jpg "Immunohistochemistry: NOL12 Antibody [NBP1-46174]")
![Immunoprecipitation: NOL12 Antibody [NBP1-46174]](https://resources.bio-techne.com/images/products/NOL12-Antibody-Immunoprecipitation-NBP1-46174-img0006.jpg "Immunoprecipitation: NOL12 Antibody [NBP1-46174]")
![Western Blot: NOL12 Antibody [NBP1-46174]](https://resources.bio-techne.com/images/products/NOL12-Antibody-Western-Blot-NBP1-46174-img0004.jpg "Western Blot: NOL12 Antibody [NBP1-46174]")
Conjugate
Catalog #
Key Product Details
Validated by
Independent Antibodies
Species Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Format
BSA Free
Concentration
1.0 mg/ml
Product Specifications
Immunogen
The immunogen for this product maps to a region between residue 163 and 213 of human Nucleolar Protein 12 using the numbering given in entry NP_077289.1 (GeneID 79159).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for NOL12 Antibody - BSA Free
Western Blot: NOL12 Antibody [NBP1-46174]
Western Blot: NOL12 Antibody [NBP1-46174] - Detection of human and mouse NOL12 by western mlot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, Jurkat, mouse TCMK-1, and mouse NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-NOL12 antibody NBP1-46174 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.![Immunohistochemistry: NOL12 Antibody [NBP1-46174]](https://resources.bio-techne.com/images/products/NOL12-Antibody-Immunohistochemistry-NBP1-46174-img0005.jpg "Immunohistochemistry: NOL12 Antibody [NBP1-46174]")
Immunohistochemistry: NOL12 Antibody [NBP1-46174]
Immunohistochemistry: NOL12 Antibody [NBP1-46174] - Detection of human NOL12 by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Affinity purified rabbit anti-NOL12 (NBP1-46174) Detection: DAB![Western Blot: NOL12 Antibody [NBP1-46174]](https://resources.bio-techne.com/images/products/NOL12-Antibody-Western-Blot-NBP1-46174-img0001.jpg "Western Blot: NOL12 Antibody [NBP1-46174]")
Western Blot: NOL12 Antibody [NBP1-46174]
Western Blot: NOL12 Antibody [NBP1-46174] - Whole cell lysate (5, 15 and 50 mcg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified rabbit anti-NOL12 antibody used for WB at 0.1 mcg/ml (A) and 1 mcg/ml (B) and used for IP at 3 mcg/mg lysate. NOL12 was also immunoprecipitated by rabbit anti-NOL12 antibody NBP1-46173, which recognizes an upstream epitope.Applications for NOL12 Antibody - BSA Free
Application
Recommended Usage
Immunohistochemistry-Paraffin
1:10-1:500
Immunoprecipitation
2-5 ug/mg lysate
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
Tris-Citrate/Phosphate (pH 7.0 - 8.0)
Format
BSA Free
Preservative
0.09% Sodium Azide
Concentration
1.0 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: NOL12
Alternate Names
dJ37E16.7, FLJ34609, MGC3731, Nop25, nucleolar protein 12, RRP17
Gene Symbol
NOL12
Additional NOL12 Products
Product Documents for NOL12 Antibody - BSA Free
Product Specific Notices for NOL12 Antibody - BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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