NOL12 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-86155
Key Product Details
Species Reactivity
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids: HQEYLKMLAEREEALEEADELDRLVTAKTESVQYDHPNHTVTVTTISDLDLSGARLLGLTPPEGGAGDRSEEEASSTEKPTKALPRKSRDPLLSQRISSLTASLHAHSRKKVKRKHPRRAQDSKKPPRAPRTSKAQRRRLTGKAR
Reactivity Notes
Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (88%), Rat (86%)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for NOL12 Antibody
Immunocytochemistry/ Immunofluorescence: NOL12 Antibody [NBP1-86155]
Immunocytochemistry/Immunofluorescence: NOL12 Antibody [NBP1-86155] - Immunofluorescent staining of human cell line U-2 OS shows localization to nucleoli. Antibody staining is shown in green.Immunohistochemistry-Paraffin: NOL12 Antibody [NBP1-86155]
Immunohistochemistry-Paraffin: NOL12 Antibody [NBP1-86155] - Staining of human cervix, uterine shows distinct nucleolar positivity in squamous epithelial cells.Applications for NOL12 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:1000 - 1:2500
Immunohistochemistry-Paraffin
1:1000 - 1:2500
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: NOL12
Alternate Names
dJ37E16.7, FLJ34609, MGC3731, Nop25, nucleolar protein 12, RRP17
Entrez Gene IDs
79159 (Human)
Gene Symbol
NOL12
UniProt
Additional NOL12 Products
Product Documents for NOL12 Antibody
Product Specific Notices for NOL12 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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