NOLC1 Antibody (3F8)
Novus Biologicals, part of Bio-Techne | Catalog # H00009221-M01
Key Product Details
Species Reactivity
Human
Applications
ELISA, Sandwich ELISA, Western Blot
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG3 Kappa Clone # 3F8
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Specifications
Immunogen
NOLC1 (NP_004732, 590 a.a. ~ 699 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. EAAKEAETPQAKKIKLQTPNTFPKRKKGEKRASSPFRRVREEEIEVDSRVADNSFDAKRGAAGDWGERANQVLKFTKGKSFRHEKTKKKRGSYRGGSISVQVNSIKFDSE
Specificity
NOLC1 - nucleolar and coiled-body phosphoprotein 1 (3F8)
Clonality
Monoclonal
Host
Mouse
Isotype
IgG3 Kappa
Scientific Data Images for NOLC1 Antibody (3F8)
![Western Blot: NOLC1 Antibody (3F8) [H00009221-M01]](https://resources.bio-techne.com/images/products/NOLC1-Antibody-3F8-Western-Blot-H00009221-M01-img0004.jpg "Western Blot: NOLC1 Antibody (3F8) [H00009221-M01]")
Western Blot: NOLC1 Antibody (3F8) [H00009221-M01]
Western Blot: NOLC1 Antibody (3F8) [H00009221-M01] - NOLC1 monoclonal antibody (M01), clone 3F8. Analysis of NOLC1 expression in HepG2.![Western Blot: NOLC1 Antibody (3F8) [H00009221-M01]](https://resources.bio-techne.com/images/products/NOLC1-Antibody-3F8-Western-Blot-H00009221-M01-img0005.jpg "Western Blot: NOLC1 Antibody (3F8) [H00009221-M01]")
Western Blot: NOLC1 Antibody (3F8) [H00009221-M01]
Western Blot: NOLC1 Antibody (3F8) [H00009221-M01] - Analysis of NOLC1 expression in transfected 293T cell line by NOLC1 monoclonal antibody (M01), clone 3F8.Lane 1: NOLC1 transfected lysate (Predicted MW: 73.7 KDa).Lane 2: Non-transfected lysate.
Sandwich ELISA: NOLC1 Antibody (3F8) [H00009221-M01] - Detection limit for recombinant GST tagged NOLC1 is approximately 1ng/ml as a capture antibody.
Applications for NOLC1 Antibody (3F8)
Application
Recommended Usage
Western Blot
1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for western blot. It has also been used for ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
IgG purified
Formulation
In 1x PBS, pH 7.4
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: NOLC1
Alternate Names
140 kDa nucleolar phosphoprotein, HCV NS5A trans-regulated protein 13, HCV NS5A-transactivated protein 13, Hepatitis C virus NS5A-transactivated protein 13, KIAA0035NS5ATP13, NOPP130, Nopp140, Nucleolar 130 kDa protein, nucleolar and coiled-body phosphoprotein 1, nucleolar and coiled-body phosphprotein 1, Nucleolar phosphoprotein p130, nucleolar protein p130, P130
Entrez Gene IDs
9221 (Human)
Gene Symbol
NOLC1
UniProt
Additional NOLC1 Products
Product Documents for NOLC1 Antibody (3F8)
Product Specific Notices for NOLC1 Antibody (3F8)
This product is produced by and distributed for Abnova, a company based in Taiwan.
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...