Key Product Details

Validated by

Independent Antibodies

Species Reactivity

Validated:

Human

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot (Negative)

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

1.0 mg/ml

View all NOLC1 Antibodies »

Product Summary for NOLC1 Antibody

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1 and 50 of human nucleolar and coiled-body phosphoprotein 1 using the numbering given in entry NP_004732.2 (GeneID 9221).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NOLC1 Antibody

Immunohistochemistry-Paraffin: NOLC1 Antibody [NBP1-22981]

Immunohistochemistry-Paraffin: NOLC1 Antibody [NBP1-22981]

Immunohistochemistry-Paraffin: NOLC1 Antibody [NBP1-22981] - Sample: FFPE section of human colon carcinoma. Antibody: Affinity purified rabbit anti- NOPP140 used at a dilution of 1:1,000 (1ug/ml). Detection: DAB
Immunoprecipitation: NOLC1 Antibody [NBP1-22981]

Immunoprecipitation: NOLC1 Antibody [NBP1-22981]

Immunoprecipitation: NOLC1 Antibody [NBP1-22981] - Detection of human NOPP140 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from K-562 cells. Antibodies: Affinity purified rabbit anti-NOPP140 antibody NBP1-22981 used for IP at 3 ug/mg lysate. NOPP140 was also immunoprecipitated by rabbit anti-NOPP140 antibody NBP1-22982, which recognizes a downstream epitope. For blotting immunoprecipitated NOPP140, NBP1-22981 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 seconds.

Applications for NOLC1 Antibody

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:500- 1:2000

Immunoprecipitation

5-15 ug/mg lysate
Application Notes
Epitope retrieval with citrate buffer pH6.0 is recommended for FFPE tissue sections
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris-Citrate/Phosphate (pH 7.0 - 8.0)

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: NOLC1

NOPP140 (140 kDa nucleolar phosphoprotein) is implicated to play a role in ribosome assembly and the regulation of rRNA transcription. It has been observed to shuttle between the nucleus and cytoplasm and may also be important to nucleolar structural organization.

Alternate Names

140 kDa nucleolar phosphoprotein, HCV NS5A trans-regulated protein 13, HCV NS5A-transactivated protein 13, Hepatitis C virus NS5A-transactivated protein 13, KIAA0035NS5ATP13, NOPP130, Nopp140, Nucleolar 130 kDa protein, nucleolar and coiled-body phosphoprotein 1, nucleolar and coiled-body phosphprotein 1, Nucleolar phosphoprotein p130, nucleolar protein p130, P130

Entrez Gene IDs

9221 (Human)

Gene Symbol

NOLC1

UniProt

NP_004732.2 (Human)

Additional NOLC1 Products

Product Documents for NOLC1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for NOLC1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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