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NOLC1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-38625

Novus Biologicals, part of Bio-Techne
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NBP2-38625
NBP2-38625-25ul

Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Summary for NOLC1 Antibody

Immunogen

This antibody was developed against a recombinant protein corresponding to amino acids: VVVSKSGSLKKRKQNEAAKEAETPQAKKIKLQTPNTFPKRKKGEKRASSPFRRVREEEIEVDSRVADNSFDAKRGAAGDWGERANQVLKF

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (87%), Rat (87%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NOLC1 Antibody

Immunocytochemistry/ Immunofluorescence: NOLC1 Antibody [NBP2-38625]

Immunocytochemistry/ Immunofluorescence: NOLC1 Antibody [NBP2-38625]

Immunocytochemistry/Immunofluorescence: NOLC1 Antibody [NBP2-38625] - Staining of human cell line HEK 293 shows localization to nucleoli fibrillar center.
Immunohistochemistry: NOLC1 Antibody [NBP2-38625]

Immunohistochemistry: NOLC1 Antibody [NBP2-38625]

Immunohistochemistry: NOLC1 Antibody [NBP2-38625] - Staining of human cerebral cortex shows moderate nucleolar positivity in neuronal cells.

Applications for NOLC1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NOLC1

NOPP140 (140 kDa nucleolar phosphoprotein) is implicated to play a role in ribosome assembly and the regulation of rRNA transcription. It has been observed to shuttle between the nucleus and cytoplasm and may also be important to nucleolar structural organization.

Alternate Names

140 kDa nucleolar phosphoprotein, HCV NS5A trans-regulated protein 13, HCV NS5A-transactivated protein 13, Hepatitis C virus NS5A-transactivated protein 13, KIAA0035NS5ATP13, NOPP130, Nopp140, Nucleolar 130 kDa protein, nucleolar and coiled-body phosphoprotein 1, nucleolar and coiled-body phosphprotein 1, Nucleolar phosphoprotein p130, nucleolar protein p130, P130

Gene Symbol

NOLC1

UniProt

Additional NOLC1 Products

Product Documents for NOLC1 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for NOLC1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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