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non-muscle Myosin IIA Antibody (4H3)

Novus Biologicals, part of Bio-Techne | Catalog # H00004627-M06

Novus Biologicals, part of Bio-Techne
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H00004627-M06

Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 4H3

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Product Specifications

Immunogen

MYH9 (NP_002464.1, 1871 a.a. ~ 1960 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. RLKQLKRQLEEAEEEAQRANASRRKLQRELEDATETADAMNREVSSLKNKLRRGDLPFVVPRRMARKGAGDGSDEEVDGKADGAEAKPAE

Specificity

myosin, heavy polypeptide 9, non-muscle

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for non-muscle Myosin IIA Antibody (4H3)

Immunocytochemistry/ Immunofluorescence: non-muscle Myosin IIA Antibody (4H3) [H00004627-M06]

Immunocytochemistry/ Immunofluorescence: non-muscle Myosin IIA Antibody (4H3) [H00004627-M06]

Immunocytochemistry/Immunofluorescence: non-muscle Myosin IIA Antibody (4H3) [H00004627-M06] - Analysis of monoclonal antibody to MYH9 on HeLa cell. Antibody concentration 10 ug/ml
Immunohistochemistry-Paraffin: non-muscle Myosin IIA Antibody (4H3) [H00004627-M06]

Immunohistochemistry-Paraffin: non-muscle Myosin IIA Antibody (4H3) [H00004627-M06]

Immunohistochemistry-Paraffin: non-muscle Myosin IIA Antibody (4H3) [H00004627-M06] - Analysis of monoclonal antibody to MYH9 on formalin-fixed paraffin-embedded human ovarian cancer. Antibody concentration 0.7 ug/ml
ELISA: non-muscle Myosin IIA Antibody (4H3) [H00004627-M06]

ELISA: non-muscle Myosin IIA Antibody (4H3) [H00004627-M06]

ELISA: non-muscle Myosin IIA Antibody (4H3) [H00004627-M06] - Detection limit for recombinant GST tagged MYH9 is approximately 0.03ng/ml as a capture antibody.

Applications for non-muscle Myosin IIA Antibody (4H3)

Application
Recommended Usage

Western Blot

1:500
Application Notes
Antibody reactivity against recombinant protein for WB. It has been used for IHC-P and ELISA.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Non-muscle Myosin IIA

The non-muscle Myosin IIA gene encodes a myosin IIA heavy chain that contains an IQ domain and a myosin head-like domain. The protein is involved in several important functions, including cytokinesis, cell motility and maintenance of cell shape. Defects in MYH9 are the cause

Long Name

Myosin-9

Alternate Names

MYH9, NMMHC II-a, NMMHC-A, NMMHC-IIA

Entrez Gene IDs

4627 (Human)

Gene Symbol

MYH9

OMIM

153640 (Human)

Additional Non-muscle Myosin IIA Products

Product Documents for non-muscle Myosin IIA Antibody (4H3)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for non-muscle Myosin IIA Antibody (4H3)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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