non-muscle Myosin IIA Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP2-62644
Key Product Details
Validated by
Independent Antibodies
Species Reactivity
Validated:
Human
Applications
Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for non-muscle Myosin IIA Antibody
Immunogen
This antibody was developed against a recombinant protein corresponding to amino acids: LEDATETADAMNREVSSLKNKLRRGDLPFVVPRRMARKGAGDGSDEEVDGKADGAEAKPA
Reactivity Notes
Mouse (88%), Rat (87%)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for non-muscle Myosin IIA Antibody
Immunohistochemistry: non-muscle Myosin IIA Antibody [NBP2-62644]
Immunohistochemistry: non-muscle Myosin IIA Antibody [NBP2-62644] - Staining of human smooth muscle shows moderate cytoplasmic positivity in muscle fibres and endothelial cells.
Immunohistochemistry: non-muscle Myosin IIA Antibody [NBP2-62644]
Immunohistochemistry: non-muscle Myosin IIA Antibody [NBP2-62644] - Staining of human kidney shows moderate to strong cytoplasmic positivity in cells in glomeruli.
Immunohistochemistry: non-muscle Myosin IIA Antibody [NBP2-62644]
Immunohistochemistry: non-muscle Myosin IIA Antibody [NBP2-62644] - Staining of human endometrium shows moderate membranous positivity in glandular cells.
![non-muscle Myosin IIA Antibody Immunocytochemistry/Immunofluorescence: non-muscle Myosin IIA Antibody [NBP2-62644]](https://resources.bio-techne.com/images/products/nbp2-62644_rabbit-polyclonal-non-muscle-myosin-iia-antibody-16320241212167.jpg "Immunocytochemistry/Immunofluorescence: non-muscle Myosin IIA Antibody [NBP2-62644]")
![non-muscle Myosin IIA Antibody Immunohistochemistry-Paraffin: non-muscle Myosin IIA Antibody Antibody [NBP2-62644]](https://resources.bio-techne.com/images/products/nbp2-62644_rabbit-polyclonal-non-muscle-myosin-iia-antibody-104202412153515.jpg "Immunohistochemistry-Paraffin: non-muscle Myosin IIA Antibody Antibody [NBP2-62644]")
Applications for non-muscle Myosin IIA Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Immunohistochemistry
1:50 - 1:200
Immunohistochemistry-Paraffin
1:50 - 1:200
Western Blot
1.0 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: Non-muscle Myosin IIA
Long Name
Myosin-9
Alternate Names
MYH9, NMMHC II-a, NMMHC-A, NMMHC-IIA
Gene Symbol
MYH9
Additional Non-muscle Myosin IIA Products
Product Documents for non-muscle Myosin IIA Antibody
Product Specific Notices for non-muscle Myosin IIA Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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