NONO Antibody (0V0N8)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-16276

Recombinant Monoclonal Antibody
Novus Biologicals, part of Bio-Techne
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NBP3-16276-100ul
NBP3-16276-20ul
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 0V0N8

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all NONO Antibodies »

Product Summary for NONO Antibody (0V0N8)

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 372-471 of human NONO (Q15233). GTFPDAREQEIRMGQMAMGGAMGINNRGAMPPAPVPAGTPAPPGPATMMPDGTLGLTPPTTERFGQAATMEGIGAIGGTPPAFNRAAPGAEFAPNKRRRY

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NONO Antibody (0V0N8)

Western Blot: NONO Antibody (0V0N8) [NBP3-16276]

Western Blot: NONO Antibody (0V0N8) [NBP3-16276]

Western Blot: NONO Antibody (0V0N8) [NBP3-16276] - Western blot analysis of extracts of various cell lines, using NONO Rabbit mAb (NBP3-16276) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
Immunocytochemistry/ Immunofluorescence: NONO Antibody (0V0N8) [NBP3-16276]

Immunocytochemistry/ Immunofluorescence: NONO Antibody (0V0N8) [NBP3-16276]

Immunocytochemistry/Immunofluorescence: NONO Antibody (0V0N8) [NBP3-16276] - Immunofluorescence analysis of U-2 OS cells using NONO Rabbit mAb (NBP3-16276) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: NONO Antibody (0V0N8) [NBP3-16276]

Immunohistochemistry-Paraffin: NONO Antibody (0V0N8) [NBP3-16276]

Immunohistochemistry-Paraffin: NONO Antibody (0V0N8) [NBP3-16276] - Immunohistochemistry of paraffin-embedded mouse brain using NONO Rabbit mAb (NBP3-16276) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
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Applications for NONO Antibody (0V0N8)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, 0.05% BSA, 50% glycerol, pH7.3

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NONO

Non-POU-domain-containing octamer binding protein (NONO) is a member of the DBHS (drosophila behavior, human splicing) domain-containing family and is an RNA- and DNA- binding protein. NONO and other DBHS domain-containing proteins are multifunctional and are reported to be involved in transcriptional regulation, mRNA processing, and DNA non-homologous end joining (NHEJ). NONO functions as a coregulator of the androgen receptor (AR) and also regulates cAMP transcriptional activity by interacting with gene promoter elements. NONO is also involved in pre-mRNA splicing through an interaction with U5 snRNA and can stimulate DNA nonhomologous end joining (NHEJ) through the interaction with ku70/G22p and ku80/XRCC5 dimers. Alternate names for NONO include 54 kDa nuclear RNA- and DNA-binding protein, p54 (nrb), 55 kDa nuclear protein, NMT55, DNA-binding p52/p100 complex, NRB54, P54, and P54NRB.

Alternate Names

NMT5552 kDa subunit, non-POU domain containing, octamer-binding, NRB54non-POU-domain-containing, octamer-binding, p54(nrb)

Gene Symbol

NONO

Additional NONO Products

Product Documents for NONO Antibody (0V0N8)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for NONO Antibody (0V0N8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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