NOP58 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-46846
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot
Cited:
Immunohistochemistry, Immunoprecipitation, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
0.2 mg/ml
Product Specifications
Immunogen
The immunogen for this product maps to a region between residue 479 and 529 of human NOP58 ribonucleoprotein homolog using the numbering given in entry NP_057018.1 (GeneID 51602).
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for NOP58 Antibody
![Immunohistochemistry-Paraffin: NOP58 Antibody [NBP1-46846]](https://resources.bio-techne.com/images/products/NOP58-Antibody-Immunohistochemistry-Paraffin-NBP1-46846-img0003.jpg "Immunohistochemistry-Paraffin: NOP58 Antibody [NBP1-46846]")
Immunohistochemistry-Paraffin: NOP58 Antibody [NBP1-46846]
Immunohistochemistry-Paraffin: NOP58 Antibody [NBP1-46846] - Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti- NOP58 used at a dilution of 1:1,000 (0.2ug/ml). Detection: DAB![Immunoprecipitation: NOP58 Antibody [NBP1-46846]](https://resources.bio-techne.com/images/products/NOP58-Antibody-Immunoprecipitation-NBP1-46846-img0001.jpg "Immunoprecipitation: NOP58 Antibody [NBP1-46846]")
Immunoprecipitation: NOP58 Antibody [NBP1-46846]
Immunoprecipitation: NOP58 Antibody [NBP1-46846] - Samples: Whole cell lysate from HeLa (5, 15 and 50 ug for WB; 1 mg for IP, 20% of IP loaded) and 293T (T; 50 ug) cells. Antibodies: Affinity purified rabbit anti-NOP58 antibody NBP1-46846 used for WB at 0.04 ug/ml (A) and 0.4 ug/ml (B) and used for IP at 3 ug/mg lysate. NOP58 was also immunoprecipitated by rabbit anti-NOP58 antibody NBP1-46845, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 10 seconds (A) and 3 seconds (B).Applications for NOP58 Antibody
Application
Recommended Usage
Immunohistochemistry
1:200- 1:1000
Immunohistochemistry-Paraffin
1:200-1:1000
Immunoprecipitation
2-5 ug/mg lysate
Western Blot
1:2000-1:10000
Application Notes
Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
TBS and 0.1% BSA
Preservative
0.09% Sodium Azide
Concentration
0.2 mg/ml
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C. Do not freeze.
Background: NOP58
Alternate Names
HSPC120, NOL5, NOP5/NOP58, NOP58 ribonucleoprotein homolog (yeast), NOP5nucleolar protein 58, Nucleolar protein 5, nucleolar protein NOP5/NOP58
Gene Symbol
NOP58
Additional NOP58 Products
Product Documents for NOP58 Antibody
Product Specific Notices for NOP58 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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