Norrin/NDP Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-77014

Novus Biologicals, part of Bio-Techne

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (93%). Backed by our 100% Guarantee.

Applications

ELISA, Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1 mg/ml

View all Norrin Antibodies »

Product Summary for Norrin/NDP Antibody - BSA Free

Immunogen

Antibody was raised against an 18 amino acid synthetic peptide from near the amino terminus of human Norrin. The immunogen is located within the first 50 amino acids of Norrin. Amino Acid Squence: DTDSKTDSSFIMDSDPRR

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Bovine: (83%)

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

15 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Norrin/NDP Antibody - BSA Free

Western Blot: Norrin/NDP AntibodyBSA Free [NBP1-77014]

Western Blot: Norrin/NDP AntibodyBSA Free [NBP1-77014]

Western Blot: Norrin/NDP Antibody [NBP1-77014] - Jurkat cell lysate with Norrin antibody at (A) 1 and (B) 2 ug/mL.
Immunocytochemistry/ Immunofluorescence: Norrin/NDP Antibody - BSA Free [NBP1-77014]

Immunocytochemistry/ Immunofluorescence: Norrin/NDP Antibody - BSA Free [NBP1-77014]

Immunocytochemistry/Immunofluorescence: Norrin/NDP Antibody [NBP1-77014] - Jurkat cells with NDP/Norrin antibody at 5 ug/ml.

Applications for Norrin/NDP Antibody - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

5 ug/ml

Western Blot

1-2 ug/ml
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Norrin

Norrie disease is an X-linked genetic disorder characterized by progressive atrophy of the eyes, mental disturbances and deafness. The gene responsible for this disease was initially identified through positional cloning. Norrin, the gene product, encodes a small secreted, cysteine-rich protein that is thought to act as a ligand for the Wnt-receptor/beta-catenin signal pathway despite having sequence homology with the Wnt family of proteins. Mice lacking this gene have abnormal blood vessel growth in the vitreous and a disorganized retina; transgenic ectopic expression of Norrin restores normal retinal vasculature. Recent evidence shows that Norrin can attenuate tPA and uPA-mediated death of transformed rat retinal ganglion cells (RGC-5) by activating the Wnt/beta-catenin pathway and regulating the phosphorylation of LRP-1, a cell surface receptor for tPA and uPA, suggesting the Norrin may function in vivo by regulating kinases which may alter the phosphorylation of LRP-1.

Alternate Names

NDP

Entrez Gene IDs

4693 (Human)

Gene Symbol

NDP

UniProt

NP_000257 (Human)

Additional Norrin Products

Product Documents for Norrin/NDP Antibody - BSA Free

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for Norrin/NDP Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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