Notch-1 Antibody [CoraFluor™ 1]

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-78292CL1

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Product Datasheet / COA / SDS

View Available Conjugates View Available Formulations

Conjugate

Catalog #

Alexa Fluor 350

NBP1-78292AF350

Alexa Fluor 405

NBP1-78292AF405

Alexa Fluor 488

NBP1-78292AF488

Alexa Fluor 532

NBP1-78292AF532

Alexa Fluor 594

NBP1-78292AF594

Alexa Fluor 647

NBP1-78292AF647

Alexa Fluor 700

NBP1-78292AF700

Alexa Fluor 750

NBP1-78292AF750

Allophycocyanin

NBP1-78292APC

Biotin

NBP1-78292B

DyLight 350

NBP1-78292UV

DyLight 405

NBP1-78292V

DyLight 488

NBP1-78292G

DyLight 550

NBP1-78292R

DyLight 594

NBP1-78292DL594

DyLight 650

NBP1-78292C

DyLight 680

NBP1-78292FR

DyLight 755

NBP1-78292IR

FITC

NBP1-78292F

HRP

NBP1-78292H

Janelia Fluor 525

NBP1-78292JF525

Janelia Fluor 549

NBP1-78292JF549

Janelia Fluor 585

NBP1-78292JF585

Janelia Fluor 635

NBP1-78292JF635

Janelia Fluor 646

NBP1-78292JF646

Janelia Fluor 669

NBP1-78292JF669

mFluor Violet 450 SE

NBP1-78292MFV450

mFluor Violet 500 SE

NBP1-78292MFV500

mFluor Violet 610 SE

NBP1-78292MFV610

PE

NBP1-78292PE

PerCP

NBP1-78292PCP

Forumulation

Catalog #

BSA Free

NBP1-78292

Product Summary
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Validated:

Human, Mouse

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Simple Western, Western Blot

Label

CoraFluor 1

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Notch-1 Antibodies »

Product Summary for Notch-1 Antibody [CoraFluor™ 1]

Immunogen

A synthetic peptide made to a C-terminal portion of the human NOTCH1 protein (between residues 2300-2350) [UniProt# P46531]

Localization

Cell membrane; Single-pass type I membrane protein. Notch 1 intracellular domain: Following proteolytical processing is translocated to the nucleus.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Description

CoraFluor(TM) 1 is a high performance terbium-based TR-FRET (Time-Resolved Fluorescence Resonance Energy Transfer) or TRF (Time-Resolved Fluorescence) donor for high throughput assay development. CoraFluor(IM) 1 absorbs UV light at approximately 340 nm, and emits at approximately 490 nm, 545 nm, 585 nm and 620 nm. It is compatible with common acceptor dyes that absorb at the emission wavelengths of CoraFluor(TM) 1. CoraFluor(TM) 1 can be used for the development of robust and scalable TR-FRET binding assays such as target engagement, ternary complex, protein-protein interaction and protein quantification assays.

Applications for Notch-1 Antibody [CoraFluor™ 1]

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Simple Western

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Preservative

No Preservative

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark. Do not freeze.

Background: Notch-1

Notch is synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase (S1 cleavage) in the trans-golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase (S3 cleavage) to release the intracellular domain (NICD) from the membrane which then translocates to the nucleus to activate transcription of downstream genes.

Alternate Names

Notch1, TAN1

Gene Symbol

NOTCH1

Additional Notch-1 Products

Product Documents for Notch-1 Antibody [CoraFluor™ 1]

Product Datasheets

Download Product Datasheets

COA

SDS

Product Specific Notices for Notch-1 Antibody [CoraFluor™ 1]

CoraFluor (TM) is a trademark of Bio-Techne Corp. Sold for research purposes only under agreement from Massachusetts General Hospital. US patent 2022/0025254

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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