Notch-2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NB600-879

Novus Biologicals, part of Bio-Techne
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC, Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Rabbit Serum

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

View all Notch-2 Antibodies »

Product Specifications

Immunogen

This whole rabbit serum was prepared by repeated immunizations with a synthetic peptide corresponding to an internal region near amino acid residues 2390-2415 of human Notch-2 (the total protein is 2471 aa). A residue of cysteine was added to the amino terminal end to facilitate coupling. (Uniprot: Q04721)

Reactivity Notes

This antiserum is directed against human Notch-2. The peptide sequence shows 100% alignment with human, dog and chimpanzee sequence. Only one (1) amino acid difference is found in mouse and this change is non-conservative. Based on the sequence we expect this antibody to react as well with rat and mouse Notch-2. No specific information is available for other reactivities.

Specificity

This antiserum is directed against human Notch-2. The peptide sequence shows 100% alignment with human, dog and chimpanzee sequence. Only one (1) amino acid difference is found in mouse and this change is non-conservative. Based on the sequence we expect this antibody to react as well with rat and mouse Notch-2. No specific information is available for other reactivities.

Clonality

Polyclonal

Host

Rabbit

Isotype

Serum

Description

This antiserum is directed against human Notch-2

Store vial at -20C prior to opening. Aliquot contents and freeze at -20C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4C as an undiluted liquid. Dilute only prior to immediate use.

Scientific Data Images for Notch-2 Antibody

Western Blot: Notch-2 Antibody [NB600-879]

Western Blot: Notch-2 Antibody [NB600-879]

Western Blot: Notch-2 Antibody [NB600-879] - Analysis of a band at 110 kDa corresponding to active Notch 2 protein (arrowhead). Analysis was performed for Notch 2 expression using 100ug of total protein lysate obtained from human mesothelial SV40 cells transfected with a plasmid encoding a constitutively active Notch 2 (intra cellular Notch 2). Lanes 1-3 contain lysate 24 h (1), 48 h (2), and 72 h (3) post transfection. Lanes 4-6 are the corresponding control cells (untransfected) taken at similar time points. The band at about 110kD represents active Notch 2. This band is not seen in the control cell. The intracellular domain of Notch 2 has a predicted band size of 110kD, corresponding to this band. Protein cell lysates were run on a 10% SDS-page gel, blotted onto Hybond C membrane, blocked overnight in PBS-Tween 20 supplemented with 5% Non-fat Milk and probed with anti-Notch 2 at a 1:400 dilution. ECL was used as visualization method.
Immunohistochemistry-Paraffin: Notch-2 Antibody [NB600-879]

Immunohistochemistry-Paraffin: Notch-2 Antibody [NB600-879]

Immunohistochemistry-Paraffin: Notch-2 Antibody [NB600-879] - Diluted 1:500 to detect Notch-2 in human kidney tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.
Notch-2 Antibody

Notch-2 Antibody

Anti-Notch 2 antibody was diluted 1:500 to detect NOTCH 2 in human kidney tissue. Tissue was formalin fixed and paraffin embedded. No pre-treatment of sample was required. The image shows the localization of antibody as the precipitated red signal, with a hematoxylin purple nuclear counter stain.

Applications for Notch-2 Antibody

Application
Recommended Usage

ELISA

1:30000-1:90000

Immunohistochemistry

1:200-1:800

Immunohistochemistry-Paraffin

1:500

Western Blot

1:400-1:2000
Application Notes
This antibody has been tested for use in ELISA and western blot. For western blot experiments, one can expect a band of ~110 kDa in size corresponding to active Notch 2 in the appropriate cell lysate. A 1:500 dilution was effective for staining FFPE human kidney tissue by Immunohistochemistry (IHC). Specific conditions for reactivity should be optimized by the end user.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Unpurified

Formulation

0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Preservative

0.1% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C short term. Aliquot and store at -80C long term. Avoid freeze-thaw cycles.

Background: Notch-2

Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C-terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membraneFunctions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBP-J kappa and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs.

Alternate Names

Notch2

Entrez Gene IDs

4853 (Human)

Gene Symbol

NOTCH2

UniProt

Q04721

Additional Notch-2 Products

Product Documents for Notch-2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Notch-2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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