Notch-2 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-19125

Novus Biologicals, part of Bio-Techne
Catalog #
Availability
Size / Price
Qty
NBP1-19125
Print Quote
Bulk Order
100% Guarantee

Key Product Details

Validated by

Independent Antibodies, Biological Validation

Species Reactivity

Human

Applications

Flow Cytometry, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

0.2 mg/ml

View all Notch-2 Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 2421 and 2471 of human neurogenic locus notch homolog protein 2 using the numbering given in entry NP_077719.2 (GeneID 4853).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Notch-2 Antibody

Western Blot: Notch-2 Antibody [NBP1-19125]

Western Blot: Notch-2 Antibody [NBP1-19125]

Western Blot: Notch-2 Antibody [NBP1-19125] - Whole cell lysate from HeLa and 293T cells. Notch2 was also immunoprecipitated by rabbit anti-Notch2 antibody NBP1-19124.
Immunohistochemistry-Paraffin: Notch-2 Antibody [NBP1-19125]

Immunohistochemistry-Paraffin: Notch-2 Antibody [NBP1-19125]

Immunohistochemistry-Paraffin: Notch-2 Antibody [NBP1-19125] - Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified rabbit anti- Notch2 used at a dilution of 1:200 (1ug/ml). Detection: DAB

Applications for Notch-2 Antibody

Application
Recommended Usage

Immunohistochemistry

1:100 - 1:500

Immunohistochemistry-Paraffin

1:100- 1:500

Immunoprecipitation

2-5 ug/mg lysate

Western Blot

1:2000-1:10000
Application Notes
Immunohistochemistry 1:100 - 1:500. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Notch-2

This gene encodes a member of the Notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signaling pathway that plays a key role in development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remain to be determined. This protein is cleaved in the trans-Golgi network, and presented on the cell surface as a heterodimer. This protein functions as a receptor for membrane bound ligands, and may play a role in vascular, renal and hepatic development. [provided by RefSeq]

Alternate Names

Notch2

Entrez Gene IDs

4853 (Human)

Gene Symbol

NOTCH2

UniProt

Q04721

Additional Notch-2 Products

Product Documents for Notch-2 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for Notch-2 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Privacy and Cookie Policy
Site Map
© Copyright 2024 Bio-Techne. All Rights Reserved.