NOV/CCN3 Antibody
Novus Biologicals, part of Bio-Techne | Catalog # NBP1-88155
Key Product Details
Species Reactivity
Validated:
Human
Applications
Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Polyclonal Rabbit IgG
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Product Summary for NOV/CCN3 Antibody
Immunogen
This antibody was developed against Recombinant Protein corresponding to amino acids:LPEPNCPAPRKVEVPGECCEKWICGPDEEDSLGGLTLAAYRPEATLGVEVSDSSVNCIEQTTEWTACSKSCGMGFSTRVTNRNRQCEMLKQTRL
Reactivity Notes
Possible cross-reactivity based on sequence identity: Mouse (81%), Rat (80%)
Clonality
Polyclonal
Host
Rabbit
Isotype
IgG
Theoretical MW
39 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for NOV/CCN3 Antibody
![Western Blot: NOV/CCN3 Antibody [NBP1-88155]](https://resources.bio-techne.com/images/products/CCN3-Antibody-Western-Blot-NBP1-88155-img0005.jpg "Western Blot: NOV/CCN3 Antibody [NBP1-88155]")
Western Blot: NOV/CCN3 Antibody [NBP1-88155]
Western Blot: NOV/CCN3 Antibody [NBP1-88155] - CCN3 Antibody [NBP1-88155] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells).
![Immunocytochemistry/ Immunofluorescence: NOV/CCN3 Antibody [NBP1-88155]](https://resources.bio-techne.com/images/products/CCN3-Antibody-Immunocytochemistry-Immunofluorescence-NBP1-88155-img0004.jpg "Immunocytochemistry/ Immunofluorescence: NOV/CCN3 Antibody [NBP1-88155]")
Immunocytochemistry/ Immunofluorescence: NOV/CCN3 Antibody [NBP1-88155]
Immunocytochemistry/Immunofluorescence: NOV/CCN3 Antibody [NBP1-88155] - CCN3 Antibody [NBP1-88155] - Staining of human cell line U-251MG shows positivity in vesicles. Antibody staining is shown in green.Applications for NOV/CCN3 Antibody
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
0.25-2 ug/ml
Western Blot
0.04-0.4 ug/ml
Application Notes
ICC/IF,Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Immunogen affinity purified
Formulation
PBS (pH 7.2) and 40% Glycerol
Preservative
0.02% Sodium Azide
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Background: NOV/CCN3
Long Name
Nephroblastoma Overexpressed Gene
Alternate Names
CCN3, IGFBP-9
Entrez Gene IDs
4856 (Human)
Gene Symbol
CCN3
UniProt
Additional NOV/CCN3 Products
Product Documents for NOV/CCN3 Antibody
Product Specific Notices for NOV/CCN3 Antibody
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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