NOX1 Antibody

Western Blot: NOX1 Antibody [NBP1-31546]

Western Blot: NOX1 Antibody [NBP1-31546] - Representative Western blots showing Nox1 and beta-tubulin in PC-3 cells or Nox4 and beta-tubulin in C2C12 cells, at 5% and 18% O2. Total cellular proteins were extracted by treating cells with NP-40 buffer (150mM NaCl, 1% NP-40, 50mM Tris-HCl pH8.0). Total protein (15ug per sample) was resolved on 10% SDS-PAGE, transferred to a polyvinylidene difluoride membrane, and probed for Nox1 or Nox4. beta-Tubulin was used as an internal loading and transfer control. All antibodies were purchased from Novus Biologicals; Nox1 (NBP1-31546), Nox4 (NB110-58851), b-tubulin (NB600-936). Data were analysed using two-tailed Student's t-tests. Bars represent means+/-SEM from at least five independent experiments. p<0.05. The identities of bands at intermediate molecular weight are unknown. Image collected and cropped by CiteAb from the following publication (hindawi.com/journals/omcl/2018/8238459/), licensed under a CC-BY license.

Western Blot: NOX1 Antibody [NBP1-31546]

Western Blot: NOX1 Antibody [NBP1-31546] - Untreated (-) and treated (+) HCT116 whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with NOX1 antibody diluted at 1:2000. The HRP-conjugated anti-rabbit IgG antibody (NBP2-19301) was used to detect the primary antibody.

Immunocytochemistry/ Immunofluorescence: NOX1 Antibody [NBP1-31546]

Immunocytochemistry/Immunofluorescence: NOX1 Antibody [NBP1-31546] - HeLa cells were fixed for 10 minutes using 10% formalin and then permeabilized for 5 minutes using 1X PBS + 0.5% Triton-X100. The cells were incubated with anti-NOX1 at 2 ug/ml overnight at 4C and detected with an anti-rabbit Dylight 488 (Green) at a 1:500 dilution. Alpha tubulin (DM1A) NB100-690 was used as a co-stain at a 1:1000 dilution and detected with an anti-mouse Dylight 550 (Red) at a 1:500 dilution. Nuclei were counterstained with DAPI (Blue). Cells were imaged using a 40X objective.