Nox4 Antibody [Biotin]

Novus Biologicals, part of Bio-Techne | Catalog # NB110-58849B

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Product Datasheet / COA / SDS

View Available Conjugates View Available Formulations

Conjugate

Catalog #

Alexa Fluor 350

NB110-58849AF350

Alexa Fluor 405

NB110-58849AF405

Alexa Fluor 488

NB110-58849AF488

Alexa Fluor 532

NB110-58849AF532

Alexa Fluor 594

NB110-58849AF594

Alexa Fluor 647

NB110-58849AF647

Alexa Fluor 700

NB110-58849AF700

Alexa Fluor 750

NB110-58849AF750

Allophycocyanin

NB110-58849APC

CoraFluor 1

NB110-58849CL1

DyLight 350

NB110-58849UV

DyLight 405

NB110-58849V

DyLight 488

NB110-58849G

DyLight 550

NB110-58849R

DyLight 594

NB110-58849DL594

DyLight 650

NB110-58849C

DyLight 680

NB110-58849FR

DyLight 755

NB110-58849IR

FITC

NB110-58849F

HRP

NB110-58849H

Janelia Fluor 525

NB110-58849JF525

Janelia Fluor 549

NB110-58849JF549

Janelia Fluor 585

NB110-58849JF585

Janelia Fluor 635

NB110-58849JF635

Janelia Fluor 646

NB110-58849JF646

Janelia Fluor 669

NB110-58849JF669

mFluor Violet 450 SE

NB110-58849MFV450

mFluor Violet 500 SE

NB110-58849MFV500

mFluor Violet 610 SE

NB110-58849MFV610

PE

NB110-58849PE

PerCP

NB110-58849PCP

Forumulation

Catalog #

BSA Free

NB110-58849

Product Specifications
Applications
Preparation & Storage
Background
Product Documents
Specific Notices

Key Product Details

Species Reactivity

Human, Mouse, Rat, Porcine, Bovine, Primate, Rabbit, Sheep

Applications

Immunoblotting, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Knockdown Validated, Western Blot

Label

Biotin

Antibody Source

Polyclonal Rabbit IgG

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Nox4 Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to an internal region of the human NOX4 protein (between residues 100-200) [UniProt Q9NPH5].

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID:32853822). Porcine reactivity reported in scientific literature (PMID: 24403605).

Localization

Endoplasmic reticulum, endoplasmic reticulum membrane, multi-pass membrane protein, cell membrane, nucleus (probable).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Applications for Nox4 Antibody [Biotin]

Application

Recommended Usage

Immunoblotting

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Knockdown Validated

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.

Application Notes

Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Nox4

NOX4 (NADPH oxidase 4) is a constitutive NADPH oxidase which complex with CYBA/p22phox for generating superoxide intracellularly and is found primarily in vascular cells, fibroblasts, osteoclasts and is abundantly expressed in the kidney. NOX4 regulates signaling cascades through phosphatases inhibition and function as oxygen sensor regulating the KCNK3/TASK-1 potassium channel as well as HIF1 alpha activity. NOX4 has also been implicated in regulation of insulin signaling cascade, apoptosis, bone resorption and LPS-mediated activation of NFkB. In vascular smooth muscle cells, NOX4 is positively regulated by Poldip2 which is a Nox4/p22phox interacting protein and Nox4/p22phox-Poldip2 complex regulates Rho-dependent cytoskeletal reorganization as well as cellular migration. TGF- beta is another positive regulator of Nox4 which is important in vascular fibrosis and differentiation. Unlike Nox1/Nox2, Nox4 is constitutively active, producing primarily H2O2 rather thanO2 and this difference in the species generated underlie NOX-specific actions in cell signaling. Nox4 contributes to basal ROS production through its constitutive activity and to increased ROS generation when stimulated by Ang II, glucose, TNF alpha and growth factors. NOX4 has been implicated in hypertension, atherosclerosis, and cardiovascular and renal complications of diabetes and in remodeling of pulmonary arteries in hypertension.

Long Name

NADPH Oxidase 4

Alternate Names

KOX-1, RENOX

Gene Symbol

NOX4

Additional Nox4 Products

Product Documents for Nox4 Antibody [Biotin]

Product Datasheets

Download Product Datasheets

COA

SDS

Product Specific Notices for Nox4 Antibody [Biotin]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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