Nox4 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NB110-58851

Novus Biologicals, part of Bio-Techne
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NB110-58851
NB110-58851SS
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Mouse, Rat, Porcine, Primate

Cited:

Human, Mouse, Rat, Porcine

Applications

Validated:

Flow (Intracellular), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Simple Western, Western Blot

Cited:

IF/IHC, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

1.0 mg/ml

View all Nox4 Antibodies »

Product Specifications

Immunogen

A synthetic peptide made to a C-terminal region (within residues 500-578) of the human NOX4 protein sequence. [Swiss-Prot# Q9NPH5].

Localization

Endoplasmic reticulum, endoplasmic reticulum membrane, multi-pass membrane protein, cell membrane, nucleus (probable).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Nox4 Antibody - BSA Free

Western Blot: Nox4 AntibodyBSA Free [NB110-58851]

Western Blot: Nox4 AntibodyBSA Free [NB110-58851]

Western Blot: Nox4 Antibody [NB110-58851] - Whole cell protein from human HeLa, Hek293, HepG2, mouse Neuro2A and rat PC12 cells was separated on a 7.5% gel by SDS-PAGE, transferred to PVDF membrane and blocked in 5% BSA in TBST. The membrane was probed with 2.0 ug/ml anti-Nox4 in 1% BSA and detected with an anti-rabbit HRP secondary antibody using chemiluminescence. Nox4 is detected at approx. 70 kDa (arrowhead)
Immunocytochemistry/ Immunofluorescence: Nox4 Antibody - BSA Free [NB110-58851]

Immunocytochemistry/ Immunofluorescence: Nox4 Antibody - BSA Free [NB110-58851]

Immunocytochemistry/Immunofluorescence: Nox4 Antibody [NB110-58851] - HeLa cells were fixed in 10% buffered formalin for 10 min and permeabilized in 0.1% Triton X-100 in PBS for 10 min. Cells were incubated with antibodies to Nox4 (NB110-58851) and the ER marker Calreticulin (NBP1-47518), each at 20 ug/ml for 1 hour at room temperature. The coverslips were washed 3x in PBS and incubated with Alexa-Fluor 488 anti-rabbit secondary antibody. (green) and DyLight 550 anti-mouse secondary antibody. The merged image shows the co-localization of Nox4 and Calreticulin in the ER.
Immunohistochemistry: Nox4 Antibody - BSA Free [NB110-58851]

Immunohistochemistry: Nox4 Antibody - BSA Free [NB110-58851]

Immunohistochemistry: Nox4 Antibody [NB110-58851] - The expression of Nox4, p21, and Rb was upregulated in degenerative discs. Immunohistochemical staining for Nox4 in NP specimens. Image collected and cropped by CiteAb from the following publication (https://www.hindawi.com/journals/omcl/2017/7426458/) licensed under a CC-BY license.
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Applications for Nox4 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

10 - 20 ug/ml

Immunohistochemistry

10 - 20 ug/ml

Immunohistochemistry-Paraffin

10 - 20 ug/ml

Immunoprecipitation

reported in scientific literature (PMID 25062272)

Western Blot

2 - 4 ug/ml
Application Notes
In Western Blot this NOX4 antibody recognizes bands at ~70kDa. See Simple Western Antibody Database for Simple Western validation: Tested in pancreas; separated by size; antibody dilution of 1:1000.

Reviewed Applications

Read 2 reviews rated 3.5 using NB110-58851 in the following applications:

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Nox4

NOX4 (NADPH oxidase 4) is a constitutive NADPH oxidase which forms a complex with CYBA/p22phox generates superoxide intracellularly, and is found primarily in vascular cells, fibroblasts, osteoclasts and is abundantly expressed in the kidney. NOX4 regulates signaling cascades through phosphatase inhibition, functions in oxygen sensor regulation in the KCNK3/TASK-1 potassium channel, and also demonstrates HIF1 alpha activity. NOX4 has also been implicated in regulation of insulin signaling cascade, apoptosis, bone resorption and LPS-mediated activation of NFkB. In vascular smooth muscle cells, NOX4 is positively regulated by Poldip2 which is a Nox4/p22phox interacting protein and Nox4/p22phox-Poldip2 complex regulates Rho-dependent cytoskeletal reorganization as well as cellular migration. TGF-beta is another positive regulator of Nox4 which is important in vascular fibrosis and differentiation. Unlike Nox1/Nox2, Nox4 is constitutively active, producing primarily H2O2 rather than O2. Nox4 contributes to basal ROS production through its constitutive activity and to increased ROS generation when stimulated by Ang II, glucose, TNF alpha and growth factors. NOX4 has been implicated in hypertension, atherosclerosis, cardiovascular and renal complications of diabetes, and in remodeling of pulmonary arteries in hypertension.

Long Name

NADPH Oxidase 4

Alternate Names

KOX-1, RENOX

Gene Symbol

NOX4

Additional Nox4 Products

Product Documents for Nox4 Antibody - BSA Free

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Nox4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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