Npas4 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-68863

Novus Biologicals, part of Bio-Techne
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NBP1-68863
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Predicted:

Bovine (100%), Canine (100%). Backed by our 100% Guarantee.

Applications

Validated:

Peptide ELISA, Western Blot

Cited:

Chromatin Immunoprecipitation (ChIP), Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG

Concentration

0.5 mg/ml

View all Npas4 Antibodies »

Product Specifications

Immunogen

Peptide with sequence CRFNTSKSLRRQS corresponding to internal region according to NP_849195.2.

Epitope

CRFNTSKSLRRQS

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Npas4 Antibody

Western Blot: Npas4 Antibody [NBP1-68863]

Western Blot: Npas4 Antibody [NBP1-68863]

Western Blot: Npas4 Antibody [NBP1-68863] - Staining of Mouse (A) and Rat (B) Brain lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
Western Blot: Npas4 Antibody [NBP1-68863]

Western Blot: Npas4 Antibody [NBP1-68863]

Western Blot: Npas4 Antibody [NBP1-68863] - NBP1-68863 (0.3ug/ml) staining of Human Frontal Cortex lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Applications for Npas4 Antibody

Application
Recommended Usage

Peptide ELISA

Detection limit 1:4000

Western Blot

0.3 - 1 ug/ml
Application Notes
WB: Approx. 90 kDa band observed in human brain (frontal cortex) lysates and in mouse and rat brain lysates (calculated MW of 87.1 kDa band according to NP_849195.2).
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

Tris saline (20 mM Tris pH 7.3, 150 mM NaCl), 0.5% BSA

Preservative

0.02% Sodium Azide

Concentration

0.5 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Npas4

NXF is a member of the basic helix-loop-helix-PER (MIM 602260)-ARNT (MIM 126110)-SIM (see SIM2; MIM 600892) (bHLH-PAS) class of transcriptional regulators, which are involved in a wide range of physiologic and developmental events (Ooe et al., 2004 [PubMed 14701734]).[supplied by OMIM]

Alternate Names

BHLHE79, bHLHe79neuronal PAS domain-containing protein 4, Class E basic helix-loop-helix protein 79, Le-PAS, neuronal PAS domain protein 4, Neuronal PAS4, NXFHLH-PAS transcription factor NXF, PASD10PAS domain-containing protein 10

Entrez Gene IDs

266743 (Human); 225872 (Mouse); 266734 (Rat)

Gene Symbol

NPAS4

UniProt

NP_849195.2 (Human)

Additional Npas4 Products

Product Documents for Npas4 Antibody

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Certificate of Analysis

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Product Specific Notices for Npas4 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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