NPIP Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-46813

Novus Biologicals, part of Bio-Techne
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NBP2-46813
NBP2-46813-25ul
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Key Product Details

Species Reactivity

Validated:

Human

Applications

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all NPIP Antibodies »

Product Summary for NPIP Antibody

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: NRMGHQPPPPTQQHSIIDNSLSLKTP

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NPIP Antibody

Western Blot: NPIP Antibody [NBP2-46813]

Western Blot: NPIP Antibody [NBP2-46813]

Western Blot: NPIPB3 Antibody [NBP2-46813] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10, Lane 2: Human cell line RT-4
Immunocytochemistry/ Immunofluorescence: NPIP Antibody [NBP2-46813]

Immunocytochemistry/ Immunofluorescence: NPIP Antibody [NBP2-46813]

Immunocytochemistry/Immunofluorescence: NPIPB3 Antibody [NBP2-46813] - Staining of human cell line A-431 shows localization to nucleoplasm.
Immunohistochemistry: NPIP Antibody [NBP2-46813]

Immunohistochemistry: NPIP Antibody [NBP2-46813]

Immunohistochemistry: NPIPB3 Antibody [NBP2-46813] - Staining of human cerebral cortex shows strong nuclear positivity in neuronal cells.

Applications for NPIP Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:20 - 1:50

Immunohistochemistry-Paraffin

1:20 - 1:50

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. Immunocytochemistry/Immunofluorescence Fixation Permeabilization: Use PFA/Triton X-100.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NPIP

The nuclear pore complex (NPC) is a very large structure made up of at least 50 different proteins that span the double membrane of the nuclear envelope functioning as a gateway for macromolecular traffic between the cytoplasm and the nucleus (Nakielny and Dreyfuss,1999). Discrete nuclear pore complex proteins or nucleoporins such as NUP98, NUP180 and p62 have been implicated in autoimmune disease and cancer. Patients with primary biliary cirrhosis (PBC) frequently produce autoantibodies against p62 and NUP180 (Wilken et al., 1993; Nesher et al., 2001) while NUP98 translocations have been found in patients with acute myelogenous leukemia (AML) (Jaju et al. 2001).

Alternate Names

FLJ42525, FLJ44848, nuclear pore complex interacting protein, nuclear pore complex-interacting protein

Entrez Gene IDs

642778 (Human)

Gene Symbol

NPIP

Additional NPIP Products

Product Documents for NPIP Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for NPIP Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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