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NPLOC4 Antibody - BSA Free

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-94655

Novus Biologicals, part of Bio-Techne
Catalog #
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NBP2-94655-0.02ml
NBP2-94655-0.1ml

Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Applications

Immunocytochemistry/ Immunofluorescence, Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Product Summary for NPLOC4 Antibody - BSA Free

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human NPLOC4 (NP_060391.2). MAESIIIRVQSPDGVKRITATKRETAATFLKKVAKEFGFQNNGFSVYINRNKTGEITASSNKSLNLLKIKHGDLLFLFPSSLAGPSSEMETSVPPGFKVFGAPNVVEDEIDQYLSKQDGKIYRSRDPQLCRHGPLGKCVHCVPLEPFDEDYLNHLEPPVKHMSFHAYIRKLTGGADKGKFVALENISCKIKSGCEGHLPWPNGICTKCQPSAITLNRQKYRHVDNIMFENHTVADRFLDFWRKTGNQHFGYLYGRYTEHKDIPLGIRAEV

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NPLOC4 Antibody - BSA Free

Western Blot: NPLOC4 AntibodyBSA Free [NBP2-94655]

Western Blot: NPLOC4 AntibodyBSA Free [NBP2-94655]

Western Blot: NPLOC4 Antibody [NBP2-94655] - Analysis of extracts of various cell lines, using NPLOC4 at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit .Exposure time: 10s.
Immunocytochemistry/ Immunofluorescence: NPLOC4 Antibody - BSA Free [NBP2-94655]

Immunocytochemistry/ Immunofluorescence: NPLOC4 Antibody - BSA Free [NBP2-94655]

Immunocytochemistry/Immunofluorescence: NPLOC4 Antibody [NBP2-94655] - Immunofluorescence analysis of U2OS cells using NPLOC4 Rabbit pAb (NBP2-94655) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunocytochemistry/ Immunofluorescence: NPLOC4 Antibody - BSA Free [NBP2-94655]

Immunocytochemistry/ Immunofluorescence: NPLOC4 Antibody - BSA Free [NBP2-94655]

Immunocytochemistry/Immunofluorescence: NPLOC4 Antibody [NBP2-94655] - Immunofluorescence analysis of L929 cells using NPLOC4 Rabbit pAb (NBP2-94655) at dilution of 1:100. Blue: DAPI for nuclear staining.

Applications for NPLOC4 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:200

Western Blot

1:1000-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: NPLOC4

The ternary complex containing UFD1L, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1L-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope

Alternate Names

FLJ20657, FLJ23742, KIAA1499nuclear protein localization protein 4 homolog, NPL4Protein NPL4, nuclear protein localization 4 homolog (S. cerevisiae)

Gene Symbol

NPLOC4

Additional NPLOC4 Products

Product Documents for NPLOC4 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for NPLOC4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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