NPM1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP1-90047

Novus Biologicals, part of Bio-Techne
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NBP1-90047-25ul
NBP1-90047
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all NPM1 Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: VAVEEDAESEDEEEEDVKLLSISGKRSAPGGGSKVPQKKVKLAADEDDDDDDEEDDDEDDDDDDFDDEEAEEKAPVKKSIRDTPAKNAQKSNQNGKDSKPSSTPRS

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (86%), Rat (86%). Human reactivity reported in scientific literature (PMID: 21738817).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for NPM1 Antibody

Western Blot: NPM1 Antibody [NBP1-90047]

Western Blot: NPM1 Antibody [NBP1-90047]

Western Blot: NPM1 Antibody [NBP1-90047] - Analysis in human cell line K562.
Immunocytochemistry/ Immunofluorescence: NPM1 Antibody [NBP1-90047]

Immunocytochemistry/ Immunofluorescence: NPM1 Antibody [NBP1-90047]

Immunocytochemistry/Immunofluorescence: NPM1 Antibody [NBP1-90047] - Staining of human cell line A-431 shows localization to nucleus & nucleoli. Antibody staining is shown in green.
Immunohistochemistry-Paraffin: NPM1 Antibody [NBP1-90047]

Immunohistochemistry-Paraffin: NPM1 Antibody [NBP1-90047]

Immunohistochemistry-Paraffin: NPM1 Antibody [NBP1-90047] - Staining of human testis shows moderate to strong nuclear positivity in cells in seminiferous ducts.
View 3 more images

Applications for NPM1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NPM1

Nucleophosmin (NPM) is a multi-functional protein that is involved in many cellular activities including cell growth, proliferation and transformation. It is frequently overexpressed in solid tumors, and has recently been found to be mutated and aberrantly localized in the cytoplasm of leukaemic blasts in patients with acute myeloid leukemia (AML).

NMP antibodies are useful tools as tumor markers in cancer research.

Long Name

Nucleophosmin/Nucleolar Phosphoprotein B23

Alternate Names

B23, Nucleophosmin, Numatrin

Gene Symbol

NPM1

Additional NPM1 Products

Product Documents for NPM1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Download SDS

Product Specific Notices for NPM1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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