NPRB/NPR2 Antibody (19C7)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26459
Recombinant monoclonal antibody expressed in HEK293F cells
Key Product Details
Species Reactivity
Validated:
Human
Applications
ELISA, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 19C7
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for NPRB/NPR2 Antibody (19C7)
Immunogen
A synthesized peptide derived from Human NPRB/NPR2 [UniProt P20594]
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for NPRB/NPR2 Antibody (19C7)
Western Blot: NPRB/NPR2 Antibody (19C7) [NBP3-26459] -
Western Blot: NPRB/NPR2 Antibody (19C7) [NBP3-26459] - Positive Western Blot detected in: HEPG2 whole cell lysate, MCF7 whole cell lysate.All lanes: NPRB/NPR2 Antibody at 1: 1000
Secondary: Goat polyclonal to rabbit IgG at 1/50000 dilution.
Predicted band size: 117 kDa
Observed band size: 117 kDa
Immunocytochemistry/Immunofluorescence: NPRB/NPR2 Antibody (19C7) [NBP3-26459] -
Immunocytochemistry/Immunofluorescence: NPRB/NPR2 Antibody (19C7) [NBP3-26459] - Staining of Hela with NPRB/NPR2 Antibody (19C7) at 1:25, counter-stained with DAPI. The cells were fixed in 4% formaldehyde and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. The secondary antibody was Alexa Fluor 501-conjugated Goat Anti-Rabbit IgG (H+L).Flow Cytometry: NPRB/NPR2 Antibody (19C7) [NBP3-26459] -
Flow Cytometry: NPRB/NPR2 Antibody (19C7) [NBP3-26459] - Overlay Peak curve showing Hela cells stained with NPRB/NPR2 Antibody (19C7) (red line) at 1:50. The cells were fixed in 4% formaldehyde and permeated by 0.2% Triton X-100. Then 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (1ug/1*10^6 cells) for 45min at 4C. The secondary antibody used was FITC-conjugated Goat Anti-rabbit IgG (H+L) at 1:200 dilution for 35min at 4C. Control antibody (green line) was rabbit IgG (1ug/1*10^6 cells) used under the same conditions. Acquisition of >10,000 events was performed.Applications for NPRB/NPR2 Antibody (19C7)
Application
Recommended Usage
Flow Cytometry
1:50-1:200
Immunocytochemistry/ Immunofluorescence
1:50-1:200
Western Blot
1:500-1:2000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, pH 7.4, 150mM NaCl and 50% glycerol
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20 to -70C. Avoid freeze-thaw cycles.
Background: NPRB/NPR2
Long Name
Natriuretic Peptide Receptor B
Alternate Names
AMDM, ANPb, ANPRB, ECDM, GC-B, GUC2B, NPR2, NPRBi, SNSK
Gene Symbol
NPR2
Additional NPRB/NPR2 Products
Product Documents for NPRB/NPR2 Antibody (19C7)
Product Specific Notices for NPRB/NPR2 Antibody (19C7)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Loading...
Loading...
Loading...
Loading...
Loading...