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NQO-1 Antibody (13A1)

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-80395

Novus Biologicals, part of Bio-Techne
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NBP2-80395

Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Human, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 13A1

Concentration

2 mg/ml

Product Summary for NQO-1 Antibody (13A1)

Immunogen

Recombinant protein within Human NQO-1 aa 1-285. (SwissProt: P15559 Human; SwissProt: P05982 Rat)

Localization

Cytoplasm.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

31 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for NQO-1 Antibody (13A1)

Western Blot: NQO-1 Antibody (13A1) [NBP2-80395]

Western Blot: NQO-1 Antibody (13A1) [NBP2-80395]

Western Blot: NQO-1 Antibody (13A1) [NBP2-80395] - Analysis of NQO-1 on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:5,000 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Mouse IgG - HRP Secondary Antibody at 1:5,000 dilution was used for 1 hour at room temperature.Positive control: Lane 1: SiHa cell lysate, untreated Lane 2: A549 cell lysate, untreated
Immunohistochemistry-Paraffin: NQO-1 Antibody (13A1) [NBP2-80395]

Immunohistochemistry-Paraffin: NQO-1 Antibody (13A1) [NBP2-80395]

Immunohistochemistry-Paraffin: NQO-1 Antibody (13A1) [NBP2-80395] - Immunohistochemical analysis of paraffin-embedded rat uterus tissue using anti-NQO-1 antibody. The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 8.0-8.4) for 20 minutes. The tissues were blocked in 5% BSA for 30 m
Immunohistochemistry-Paraffin: NQO-1 Antibody (13A1) [NBP2-80395]

Immunohistochemistry-Paraffin: NQO-1 Antibody (13A1) [NBP2-80395]

Immunohistochemistry-Paraffin: NQO-1 Antibody (13A1) [NBP2-80395] - Analysis in human kidney tissue using NQO-1 antibody. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) for 20 minutes. The tissues were blocked in 5% BSA for 30 minutes at room temperature, washed with

Applications for NQO-1 Antibody (13A1)

Application
Recommended Usage

Immunohistochemistry-Paraffin

1:50-1:100

Western Blot

1:1000-1:5000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH7.4), 0.2% BSA, 50% Glycerol

Preservative

0.05% Sodium Azide

Concentration

2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: NQO-1

NQO1 (NAD(P)H dehydrogenase [quinone] 1) is a 2-electron reductase that detoxifies quinones derived from the oxidation of phenolic metabolites of benzene. Individuals homozygous for the T/T form of the 609C-T polymorphism have an increased risk of benzene hematotoxicity.

Long Name

NAD(P)H Dehydrogenase, Quinone 1

Alternate Names

Azoreductase, DHQU, DIA4, Diaphorase-4, Dioxin-inducible 1, DTD, Menadione Reductase, NMOR1, NQO1, QR1, Quinone Reductase 1

Gene Symbol

NQO1

Additional NQO-1 Products

Product Documents for NQO-1 Antibody (13A1)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for NQO-1 Antibody (13A1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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