NQO-1 Antibody (9M3H10)
Novus Biologicals, part of Bio-Techne | Catalog # NBP3-15793
Recombinant Monoclonal Antibody
Key Product Details
Validated by
Knockout/Knockdown
Species Reactivity
Validated:
Human, Mouse, Rat
Applications
Immunocytochemistry/ Immunofluorescence, Knockdown Validated, Western Blot
Label
Unconjugated
Antibody Source
Recombinant Monoclonal Rabbit IgG Clone # 9M3H10
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Product Summary for NQO-1 Antibody (9M3H10)
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human NQO-1 (P15559). MVGRRALIVLAHSERTSFNYAMKEAAAAALKKKGWEVVESDLYAMNFNPIISRKDITGKLKDPANFQYPAESVLAYKEGHLSPDIVAEQKKLEAADLVIF
Clonality
Monoclonal
Host
Rabbit
Isotype
IgG
Scientific Data Images for NQO-1 Antibody (9M3H10)
NQO-1 Antibody (1O2N3) [NBP3-15793] - Analysis of extracts of various lysates, using NQO1 antibody at 1:20000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
NQO-1 Antibody (1O2N3) [NBP3-15793] - Analysis of extracts of various lysates, using NQO1 antibody at 1:20000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
NQO-1 Antibody (1O2N3) [NBP3-15793] - Analysis of extracts from wild type(WT) and NQO1 knockdown (KD) HeLa cells, using NQO1 antibody at 1:20000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
NQO-1 Antibody (1O2N3) [NBP3-15793] - Analysis of extracts from wild type(WT) and NQO1 knockdown (KD) HeLa cells, using NQO1 antibody at 1:20000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
NQO-1 Antibody (1O2N3) [NBP3-15793] - Analysis of Rat stomach using NQO1 antibody at dilution of 1:300 (40x lens).Blue: DAPI for nuclear staining.
NQO-1 Antibody (1O2N3) [NBP3-15793] - Analysis of Rat stomach using NQO1 antibody at dilution of 1:300 (40x lens).Blue: DAPI for nuclear staining.Applications for NQO-1 Antibody (9M3H10)
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100 -1:500
Western Blot
1:5000 - 1:20000
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Affinity purified
Formulation
PBS, 0.05% BSA, 50% glycerol, pH7.3
Preservative
0.02% Sodium Azide
Concentration
Please see the vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at -20C. Avoid freeze-thaw cycles.
Background: NQO-1
Long Name
NAD(P)H Dehydrogenase, Quinone 1
Alternate Names
Azoreductase, DHQU, DIA4, Diaphorase-4, Dioxin-inducible 1, DTD, Menadione Reductase, NMOR1, NQO1, QR1, Quinone Reductase 1
Gene Symbol
NQO1
Additional NQO-1 Products
Product Documents for NQO-1 Antibody (9M3H10)
Product Specific Notices for NQO-1 Antibody (9M3H10)
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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