NQO-1 Antibody (A180) [FITC]

Novus Biologicals, part of Bio-Techne | Catalog # NB200-209F

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Canine, Primate

Applications

Flow (Intracellular), Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Frozen, Immunohistochemistry-Paraffin, Immunoprecipitation, Simple Western, Western Blot

Label

FITC (Excitation = 495 nm, Emission = 519 nm)

Antibody Source

Monoclonal Mouse IgG1 Clone # A180

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all NQO-1 Antibodies »

Product Summary for NQO-1 Antibody (A180) [FITC]

Immunogen

Full length recombinant NQO1 from human lung [UniProt# P15559]

Reactivity Notes

Mouse reactivity reported in scientific literature (PMID: 24475200)

Localization

Cytoplasmic (primarily), Nuclear (faintly)

Specificity

Does not cross-react with NQO2.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for NQO-1 Antibody (A180) [FITC]

Application
Recommended Usage

Flow (Intracellular)

Optimal dilutions of this antibody should be experimentally determined.

Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Frozen

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilutions of this antibody should be experimentally determined.

Simple Western

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: NQO-1

NAD(P)H:quinone oxidoreductase (NQO) is a ubiquitous flavoprotein enzyme which catalyzes the two-electron reduction/detoxification of quinones/quinones derivatives and it has two members - NQO1 (alias DT-diaphorase) which uses NADPH as a source of electrons, and NQO2 (NRH:quinone oxidoreductase 2) which employs dihydronicotinamide riboside (NRH) as electron donor. Expressed as cytosolic enzyme with maximum levels in liver, NQO1 is highly inducible by oxidants, electrophiles, phenolic compounds etc and its expression is regulated by Keap1/Nrf2/ARE pathway in mammals. NQO1 gene promotor contains multiple AREs (antioxidant response elements) and a functional XRE (xenobiotic response element), and AhR binding to XRE is critical for NQO1 induction by xenobiotics. Moreover, Nrf2/ARE and AhR/XRE have been proposed to coordinate in NQO1 expression regulation. Besides biotransformation, NQO1 also implicates in the maintenance of endogenous lipid-soluble antioxidants alpha-tocopherol and ubiquinone in their reduced/active forms, direct scavenging of superoxide anion radicals, stabilization of the tumor suppressor proteins including p53 and as a gatekeeper of the 20S proteasome pathway.

Long Name

NAD(P)H Dehydrogenase, Quinone 1

Alternate Names

Azoreductase, DHQU, DIA4, Diaphorase-4, Dioxin-inducible 1, DTD, Menadione Reductase, NMOR1, NQO1, QR1, Quinone Reductase 1

Gene Symbol

NQO1

Additional NQO-1 Products

Product Documents for NQO-1 Antibody (A180) [FITC]

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for NQO-1 Antibody (A180) [FITC]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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