NRAGE/MAGED1 Antibody (1E1)

Novus Biologicals, part of Bio-Techne | Catalog # H00009500-M06

Novus Biologicals, part of Bio-Techne
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2b Kappa Clone # 1E1

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all NRAGE Antibodies »

Product Specifications

Immunogen

MAGED1 (NP_001005333, 117 a.a. ~ 226 a.a) partial recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. EMADIQVSAAAARPKSAFKVQNATTKGPNGVYDFSQAHNAKDVPNTQPKAAFKSQNATPKGPNAAYDFSQAATTGELAANKSEMAFKAQNATTKVGPNATYNFSQSLNAN

Specificity

MAGED1 - melanoma antigen family D, 1 (1E1)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2b Kappa

Scientific Data Images for NRAGE/MAGED1 Antibody (1E1)

Western Blot: NRAGE/MAGED1 Antibody (1E1) [H00009500-M06]

Western Blot: NRAGE/MAGED1 Antibody (1E1) [H00009500-M06]

Western Blot: NRAGE/MAGED1 Antibody (1E1) [H00009500-M06] - Analysis of MAGED1 expression in Jurkat (Cat # L017V1).
Immunohistochemistry-Paraffin: NRAGE/MAGED1 Antibody (1E1) [H00009500-M06]

Immunohistochemistry-Paraffin: NRAGE/MAGED1 Antibody (1E1) [H00009500-M06]

Immunohistochemistry-Paraffin: NRAGE/MAGED1 Antibody (1E1) [H00009500-M06] - Analysis of monoclonal antibody to MAGED1 on formalin-fixed paraffin-embedded human esophagus. Antibody concentration 3 ug/ml
ELISA: NRAGE/MAGED1 Antibody (1E1) [H00009500-M06]

ELISA: NRAGE/MAGED1 Antibody (1E1) [H00009500-M06]

ELISA: NRAGE/MAGED1 Antibody (1E1) [H00009500-M06] - Detection limit for recombinant GST tagged MAGED1 is approximately 1ng/ml as a capture antibody.

Applications for NRAGE/MAGED1 Antibody (1E1)

Application
Recommended Usage

ELISA

1:100-1:2000

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:500
Application Notes
Antibody reactive against cell lysate and recombinant protein for western blot. It has also been used for ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: NRAGE

This gene is a member of the melanoma antigen gene (MAGE) family. Most of the genes of this family encode tumor specific antigens that are not expressed in normal adult tissues except testis. Although the protein encoded by this gene shares strong homology with members of the MAGE family, it is expressed in almost all normal adult tissues. This gene has been demonstrated to be involved in the p75 neurotrophin receptor mediated programmed cell death pathway. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq]

Long Name

Neurotrophin Receptor-interacting MAGE Homolog

Alternate Names

Dlxin-1, MAGED1

Entrez Gene IDs

9500 (Human)

Gene Symbol

MAGED1

UniProt

NP_001005333 (Human)

Additional NRAGE Products

Product Documents for NRAGE/MAGED1 Antibody (1E1)

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

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Product Specific Notices for NRAGE/MAGED1 Antibody (1E1)

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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