Nrf1 Antibody

Novus Biologicals, part of Bio-Techne | Catalog # NBP2-19586

Novus Biologicals, part of Bio-Techne
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NBP2-19586
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat

Cited:

Mouse

Predicted:

Bovine (100%), Chicken (99%), Xenopus (93%), Zebrafish (92%). Backed by our 100% Guarantee.

Applications

Validated:

Immunocytochemistry/ Immunofluorescence, Immunohistochemistry, Immunohistochemistry-Paraffin, Immunoprecipitation, Western Blot

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

View all Nrf1 Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Nrf1. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (93%).

Localization

Nucleus

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

54 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Nrf1 Antibody

Western Blot: Nrf1 Antibody [NBP2-19586]

Western Blot: Nrf1 Antibody [NBP2-19586]

Western Blot: Nrf1 Antibody [NBP2-19586] - A. 30 ug 293T whole cell lysate/extractB. 30 ug 293T nuclear lysate/extract C. 30 ug A431 whole cell lysate/extract | D. 30 ug A431 nuclear lysate/extract E. 30 ug HeLa whole cell lysate/extract F. 30 ug HeLa nuclear lysate/extract G. 30 ug HepG2 whole cell lysate/extract H. 30 ug HepG2 nuclear lysate/extract10 % SDS-PAGE NRF1 antibody dilution: 1:1000
Immunohistochemistry-Paraffin: Nrf1 Antibody [NBP2-19586]

Immunohistochemistry-Paraffin: Nrf1 Antibody [NBP2-19586]

Immunohistochemistry-Paraffin: Nrf1 Antibody [NBP2-19586] - Paraffin-embedded mouse colon. NRF1 antibody dilution: 1:500.
Western Blot: Nrf1 Antibody [NBP2-19586]

Western Blot: Nrf1 Antibody [NBP2-19586]

Western Blot: Nrf1 Antibody [NBP2-19586] - IP samples: 293T whole cell extract A. Control with 4 ug of preimmune Rabbit IgG B. Immunoprecipitation of NRF1 protein by 4 ug NRF1 antibody. 10 % SDS-PAGE The immunoprecipitated NRF1 protein was detected by NRF1 antibody diluted at 1:1000. [EasyBlot anti-rabbit IgG was used as a secondary reagent].
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Applications for Nrf1 Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunohistochemistry

1:100-1:1000

Immunohistochemistry-Paraffin

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:3000
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Nrf1

This gene encodes a protein that homodimerizes and functions as a transcription factor which activates the expression of some key metabolic genes regulating cellular growth and nuclear genes required for respiration, heme biosynthesis, and mitochondrial DNA transcription and replication. The protein has also been associated with the regulation of neurite outgrowth. Alternate transcriptional splice variants, which encode the same protein, have been characterized. Additional variants encoding different protein isoforms have been described but they have not been fully characterized. Confusion has occurred in bibliographic databases due to the shared symbol of NRF1 for this gene and for "nuclear factor (erythroid-derived 2)-like 1" which has an official symbol of NFE2L1. [provided by RefSeq]

Long Name

NF-E2-related Factor 1

Alternate Names

HBZ17, LCR-F1, NFE2L1, TCF11

Entrez Gene IDs

4899 (Human)

Gene Symbol

NRF1

Additional Nrf1 Products

Product Documents for Nrf1 Antibody

Product Datasheets

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot number in the search box below.

Product Specific Notices for Nrf1 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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