Nrf2 Antibody (2D12)

Novus Biologicals, part of Bio-Techne | Catalog # NBP3-26113

Recombinant monoclonal antibody expressed in HEK293F cells
Novus Biologicals, part of Bio-Techne
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NBP3-26113-100ul
NBP3-26113-50ul
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Key Product Details

Species Reactivity

Human

Applications

ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 2D12

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

View all Nrf2 Antibodies »

Product Specifications

Immunogen

A synthesized peptide derived from Human Nrf2 [UniProt Q16236]

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Nrf2 Antibody (2D12)

Nrf2 Antibody (2D12)

Immunocytochemistry/Immunofluorescence: Nrf2 Antibody (2D12) [NBP3-26113] -

Immunocytochemistry/Immunofluorescence: Nrf2 Antibody (2D12) [NBP3-26113] - Staining of Hela Cells with Nrf2 Antibody (2D12) at 1:50, counter-stained with DAPI. The cells were fixed in 4% formaldehyde, permeated by 0.2% Triton X-100, and blocked in 10% normal Goat Serum. The cells were then incubated with the antibody overnight at 4C. Nuclear DNA was labeled in blue with DAPI. The secondary antibody was FITC-conjugated Goat Anti-Rabbit IgG (H+L).

Applications for Nrf2 Antibody (2D12)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:20-1:200
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS, pH 7.4, 150mM NaCl and 50% glycerol

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20 to -70C. Avoid freeze-thaw cycles.

Background: Nrf2

Nrf2 is a transcription factor which belongs to the Cap N Collar (CNC-bZIP) subfamily with an implication in adaptive responses to oxidative stress. Nrf2 is a basic leucine zipper transcription factor that can bind to a NF-E2/AP-1 repeat sequence in the promoter of the beta-globin gene and has two types of binding partners, a cytoplasmic repressor (Keap I) and multiple nuclear binding partners. During oxidative stress on a cell, Nrf2 is released from Keap I, possibly via PKC phosphorylation at serine 40, and translocates to the nucleus. In the nucleus, Nrf2 binds to antioxidant responsive element (ARE) and leads to transcriptional activation of downstream genes for GSTs, glutamate-cysteine ligase, HO-1, and thioredoxin. Nrf2 and its downstream proteins have shown to have important functions in protection against oxidative stress or chemical-induced cellular damage in liver and lung, prevention in cancer formation in GI tract, and promotion of the wound-healing process.

Long Name

NF-E2-related Factor 2

Alternate Names

NFE2L2

Gene Symbol

NFE2L2

Additional Nrf2 Products

Product Documents for Nrf2 Antibody (2D12)

Product Datasheets

Certificate of Analysis

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Product Specific Notices for Nrf2 Antibody (2D12)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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